RNA Interference-based Suppression of Phosphoenolpyruvate Carboxylase Results in Susceptibility of Rapeseed to Osmotic Stress
RNA Interference-based Suppression of Phosphoenolpyruvate Carboxylase Results in Susceptibility of Rapeseed to Osmotic Stress
复制标题
基于 RNA 干扰的磷酸烯醇丙酮酸羧化酶抑制导致油菜籽对渗透胁迫的敏感性
DOI：
10.1111/j.1744-7909.2010.00948.x
复制
发表时间：
2010-06-01
影响因子：
11.4
通讯作者：
Xu, Yinong
中科院分区：
文献类型：
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作者：
Chen, Mei;Tang, Yunlai;Xu, Yinong
磷酸烯醇式丙酮酸羧化酶(PEPCase；EC 4.1.1.31)在C-3植物中的不同功能还没有在C-4植物中得到很好的理解。研究PEPCase在C-3植物甘蓝型油菜(Brassica napus L.)中的功能。利用RNA干扰(RNAi)技术沉默PEPCase基因(简称BNPE15)。在正常生长条件下，转基因油菜和野生型油菜的脂肪含量和脂肪酸组成没有显著差异。然而，在渗透胁迫下，转基因植株根和叶片的膜透性和膜脂过氧化作用均高于对照植株。这表明转基因植株比野生型植株更容易受到渗透胁迫的影响。综上所述，这些结果表明，RNAi抑制PEPCase导致油菜对渗透胁迫的敏感性，PEPCase参与了C-3植物对环境胁迫的反应。
The diverse functions of phosphoenolpyruvate carboxylase (PEPCase; EC 4.1.1.31) in C-3 plants are not as well understood as in C-4 plants. To investigate the functions of PEPCase in C-3 plants, rapeseed (Brassica napus L.) PEPCase gene (referred to as BNPE15) was silenced by the RNA interference (RNAi) technique. Under normal growth conditions, no significant difference in lipid content and fatty acid composition were found between wild-type (WT) and transgenic rapeseed plants. However, when these plants were subjected to osmotic stress induced by osmoticum polyethylene glycol (PEG-6000), membrane permeability and membrane lipid peroxidization in roots and leaves of transgenic plants were higher than those of WT plants. It suggested that transgenic plants are more susceptible to osmotic stress than WT plants. Taken together, the results showed that the suppression of PEPCase by RNAi leads to susceptibility to osmotic stress in rapeseed, and PEPCase is involved in the response of C-3 plants to environmental stress.