Influence of apple phytochemicals in ZnO nanoparticles formation, photoluminescence and biocompatibility for biomedical applications
Influence of apple phytochemicals in ZnO nanoparticles formation, photoluminescence and biocompatibility for biomedical applications
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DOI：
10.1016/j.msec.2019.03.084
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发表时间：
2019-08-01
影响因子：
7.9
通讯作者：
Montemor, Maria F.
中科院分区：
文献类型：
--
作者：
Alvesa, Marta M.;Andrade, Suzana M.;Montemor, Maria F.
使用苹果（变种 Starking）植物化学物质新型绿色合成的 ZnO 纳米颗粒 (NP) 为生物成像应用提供了巨大的潜力。与用纯植物化学物质（槲皮素或蔗糖）和水合成的 ZnO 微粒相比，这些纳米颗粒的尺寸和形状很大程度上取决于所使用的有机前体。基于这些发现，提出了使用苹果植物化学物质合成 ZnO NP 的新见解。以稳态和时间分辨光致发光测量为特征的光致发光特性表明，除了所有颗粒观察到的强烈尖锐的近紫外带边缘发射之外，在亚纳秒寿命下，还检测到微粒在 2.20 eV 处的强宽发射带峰，较长的衰减时间与晶体缺陷相关。此外，通过荧光寿命成像显微镜 (FLIM) 进一步探索了 ZnO 颗粒的光致发光特性，表明其足以用于成像应用。在人真皮成纤维细胞中评估的细胞毒性，通过不受影响的细胞活力、总线粒体活性和 F-肌动蛋白细胞骨架组织证明了 ZnO NP 的生物安全性，与微粒所描述的一定程度的细胞毒性形成鲜明对比。讨论了植物化学物质对 ZnO 细胞毒性的影响。据作者所知，这是用苹果提取物合成 ZnO NP 的第一篇报道。选择广泛用于食品工业的水果的新颖性将通过循环经济的概念提供负担得起的NP。这些 ZnO 纳米颗粒经过验证的生物安全性及其固有的光致发光特性，为开发具有成本效益的生物成像材料开辟了前景，并有可能进一步应用于生物医学应用。
The new green-synthesised ZnO nanoparticles (NPs) using apple (var. Starking) phytochemicals present a great potential for bioimaging applications. These NPs, when compared with ZnO microparticles synthesised with pure phytochemicals (quercetin or sucrose), and water, revealed that sizes and shapes were widely dependent on the organic precursors used. Based on these findings, new insights into the synthesis of ZnO NPs using apple phytochemicals were presented.The photoluminescent properties, characterized by steady-state and time resolve photoluminescence measurements, revealed that besides the intense sharp near-UV band edge emission observed for all particles, with sub-nanosecond lifetime, a strong broad emission band peak at 2.20 eV was detected for microparticles, with longer decay times being associate to crystal defects. Additionally, the photoluminescent properties of ZnO particles, further explored by fluorescence lifetime imaging microscopy (FLIM), suggested adequacy for imaging applications. The cytotoxicity, evaluated in human dermal fibroblasts, proving the biosafety of ZnO NPs by an unaffected cellular viability, total mitochondrial activity and F-actin cytoskeleton organization, contrasted with some degree of cytotoxicity depicted for microparticles. The influence of the phytochemicals in ZnO cytotoxicity was discussed.To the authors' best knowledge this is the first report of ZnO NPs synthesised with apple extracts. The novelty of choosing a fruit widely used in the food industry will render affordable NPs through the concept of circular economy. The proved biosafety of these ZnO NPs together with their intrinsic photoluminescent properties, open perspectives for the development of cost-effective bioimaging materials with potential to be further directed into biomedical applications.