MicroRNA-124 negatively regulates chloride intracellular channel 1 to suppress the migration and invasion of liver cancer cells
MicroRNA-124 negatively regulates chloride intracellular channel 1 to suppress the migration and invasion of liver cancer cells
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DOI：
10.3892/or.2019.7250
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发表时间：
2019-07
期刊：
影响因子：
4.2
通讯作者：
Xupeng Yue;Yuanyuan Cui;Q. You;Yanxin Lu;Ju-feng Zhang
中科院分区：
文献类型：
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作者：
Xupeng Yue;Yuanyuan Cui;Q. You;Yanxin Lu;Ju-feng Zhang
MicroRNAs(MiRNAs)的失调与包括肝癌在内的多种癌症的发生和发展有关。MiRNA(MiR)-124在肝癌中的异常表达已被证实，但其在肝癌中的作用机制尚不清楚。肝癌转移是最常见的死亡原因之一。本研究表明miR-124对肝癌细胞的增殖、迁移和侵袭均有抑制作用。此外，细胞内氯离子通道1(CLIC1)被确定为miR-124在肝癌细胞中的新靶点。在肝癌细胞中，miR-124的过表达降低了CLIC1在蛋白质和mRNA水平的表达。CLIC1表达下调可减少肝癌细胞的迁移和侵袭，但不影响细胞增殖。综上所述，这些结果表明CLIC1是miR-124介导的抑制细胞迁移和侵袭作用的关键靶点。因此，miR-124或抑制CLIC1可能对人肝癌的治疗具有诊断价值和治疗潜力。
The dysregulation of microRNAs (miRNAs) is associated with the development and progression of a variety of cancers, including liver cancer. Aberrant expression of miRNA (miR)-124 has been demonstrated in liver cancer, but its functional mechanism in liver cancer is still largely unknown. Metastasis of liver cancer is one of the most common causes of mortality. The present study showed that miR-124 inhibited the proliferation, migration and invasion of liver cancer cells. Furthermore, chloride intracellular channel 1 (CLIC1) was identified as a novel target of miR-124 in liver cancer cells. Overexpression of miR-124 reduced CLIC1 expression at both the protein and mRNA levels in liver cancer cells. Downregulation of CLIC1 decreased the migration and invasion of liver cancer cells without affecting cell proliferation. Taken together, these results showed that CLIC1 is a critical target for miR-124-mediated inhibitory effects on cell migration and invasion. Thus, miR-124 or suppression of CLIC1 may have diagnostic value and therapeutic potential for the treatment of human liver cancer.