Ingestion of a moderate high-sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon-like peptide-1 secretion
Ingestion of a moderate high-sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon-like peptide-1 secretion
复制标题
DOI：
10.1111/j.2040-1124.2012.00208.x
复制
发表时间：
2012-10-01
影响因子：
3.2
通讯作者：
Ozaki, Nobuaki
中科院分区：
文献类型：
--
作者：
Sakamoto, Eriko;Seino, Yusuke;Ozaki, Nobuaki
目的/简介：过量摄取蔗糖会导致严重的健康问题，例如糖尿病。在动物研究中，高糖饮食（SUC）的消耗已被证明会导致肥胖，胰岛素抵抗和葡萄糖耐受不良。然而，已经进行了几项体内实验，使用的饮食中蔗糖含量（占总热量的5070%）比人类通常摄入的高得多。在本研究中，我们研究了适度的SUC对葡萄糖代谢的影响及其潜在机制。材料与方法：C57BL/6J小鼠分别给予SUC（38.5%蔗糖）、高淀粉日粮（ST）或对照日粮5周。我们评估了葡萄糖耐量、肠促胰岛素分泌和肝脏葡萄糖代谢。结果：口服葡萄糖耐量试验（OGTT）显示，sc喂养的小鼠早期血浆葡萄糖水平明显高于st喂养的小鼠或对照小鼠，血浆胰岛素水平在任何阶段都没有变化。suc喂养的小鼠表现出胰岛素敏感性的显著改善。胰高血糖素样肽-1 （Glucagon-like peptide-1, GLP-1）的分泌在口服葡萄糖给药15分钟后显著低于st喂养或对照小鼠。肝葡萄糖激酶（GCK）活性显著降低。在OGTT过程中，糖原在肝脏中的积累以一种时间依赖性的方式被抑制。结论：这些结果表明，摄入适量SUC的小鼠表现出葡萄糖耐受不良，肝脏GCK活性降低，GLP-1分泌受损。[J] .糖尿病投资杂志，doi: 10.1111/ J .2040-1124.2012.00208。x, 2012)
Aims/Introduction: Excessive intake of sucrose can cause severe health issues, such as diabetes mellitus. In animal studies, consumption of a high-sucrose diet (SUC) has been shown to cause obesity, insulin resistance and glucose intolerance. However, several in vivo experiments have been carried out using diets with much higher sucrose contents (5070% of the total calories) than are typically ingested by humans. In the present study, we examined the effects of a moderate SUC on glucose metabolism and the underlying mechanism. Materials and Methods: C57BL/6J mice received a SUC (38.5% sucrose), a high-starch diet (ST) or a control diet for 5 weeks. We assessed glucose tolerance, incretin secretion and liver glucose metabolism. Results: An oral glucose tolerance test (OGTT) showed that plasma glucose levels in the early phase were significantly higher in SUC-fed mice than in ST-fed or control mice, with no change in plasma insulin levels at any stage. SUC-fed mice showed a significant improvement in insulin sensitivity. Glucagon-like peptide-1 (GLP-1) secretion 15 min after oral glucose administration was significantly lower in SUC-fed mice than in ST-fed or control mice. Hepatic glucokinase (GCK) activity was significantly reduced in SUC-fed mice. During the OGTT, the accumulation of glycogen in the liver was suppressed in SUC-fed mice in a time-dependent manner. Conclusions: These results indicate that mice that consume a moderate SUC show glucose intolerance with a reduction in hepatic GCK activity and impairment in GLP-1 secretion. (J Diabetes Invest, doi: 10.1111/j.2040-1124.2012.00208.x, 2012)