Identification of PTPRσ-interacting proteins by proximity-labeling assay.
Identification of PTPRσ-interacting proteins by proximity-labeling assay.
复制标题
通过邻近标记测定鉴定 PTPRσ 相互作用蛋白。
DOI：
--
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
K. Sakamoto
中科院分区：
文献类型：
--
作者：
Yuanhao Gong;Shaniya Abudureyimu;K. Kadomatsu;K. Sakamoto
受体蛋白酪氨酸磷酸酶（RPTP）是 I 型跨膜蛋白，参与多种生物学和病理过程。它们的功能应该是通过其特定底物的酪氨酸去磷酸化来发挥的。然而，我们对 RPTP 的特定底物或相互作用蛋白的全面了解还很有限。 PTPRσ 属于 2a 类 RPTP 家族，使皮质蛋白去磷酸化，并响应其配体硫酸软骨素导致自噬通量破坏和轴突再生抑制。在这里，我们将邻近依赖性生物素识别（BioID）测定（一种邻近标记测定）应用于 PTPRσ，并可重复地鉴定出 99 个候选物作为 PTPRσ 的相互作用物，包括已知的相互作用物，例如 Liprin-α 和 Trio。值得注意的是，皮质素也列在我们的测定中。我们的结果表明，BioID 测定是鉴定 RPTP 相互作用蛋白（包括其特定底物）的强大而可靠的工具。
Receptor protein tyrosine phosphatases (RPTPs) are type-I transmembrane proteins and involved in various biological and pathological processes. Their functions are supposed to be exerted through tyrosine dephosphorylation of their specific substrates. However, our comprehensive understanding of specific substrates or interacting proteins for RPTPs is poor. PTPRσ belongs to class 2a RPTP family, dephosphorylates cortactin, and leads to autophagy flux disruption and axonal regeneration inhibition in response to its ligand chondroitin sulfate. Here, we applied proximity-dependent biotin identification (BioID) assay, a proximity labeling assay, to PTPRσ and reproducibly identified the ninety-nine candidates as interactors for PTPRσ including already-known interactors such as Liprin-α and Trio. Of note, cortactin was also listed up in our assay. Our results suggest that the BioID assay is a powerful and reliable tool to identify RPTP-interacting proteins including its specific substrate.
DOI：
10.1073/pnas.93.11.5466
发表时间：
1996-05-28
影响因子：
11.1
作者：
Debant, A;SerraPages, C;Streuli, M
通讯作者：
Streuli, M
DOI：
10.1073/pnas.94.5.1680
发表时间：
1997-03-04
影响因子：
11.1
作者：
Flint, AJ;Tiganis, T;Tonks, NK
通讯作者：
Tonks, NK