Neurodegeneration in the rat hippocampus and striatum after middle cerebral artery occlusion
Neurodegeneration in the rat hippocampus and striatum after middle cerebral artery occlusion
复制标题
DOI：
10.1016/s0006-8993(01)03371-6
复制
发表时间：
2002-03-08
期刊：
影响因子：
2.9
通讯作者：
Pennypacker, KR
中科院分区：
文献类型：
--
作者：
Butler, TL;Kassed, CA;Pennypacker, KR
缺血的动物模型被广泛用于阐明人类心血管疾病导致的脑损伤的分子机制。我们已经使用荧光阴离子染料Fluoro-Jade来检查与大脑中动脉闭塞（MCAO）模型相关的细胞变性。MCAO导致皮质梗死以及海马损伤，导致海马神经元的延迟性死亡。分别于伤后6 h、12 h、1、4、7、14、21 d取脑组织行病理切片检查。Fluoro-Jade标记的纹状体被认为是在一个漫长的时间过程中，与损伤后6小时开始退化。相反，海马中的神经元变性发生在损伤后12小时至7天之间，神经元死亡在4天达到高峰。GFAP/Fluoro-Jade双标记显示，在纹状体的晚期时间点，Fluoro-Jade阳性染色包括星形胶质细胞。总之，结果表明Fluoro-Jade是缺血性损伤后细胞变性的有用标志物。此外，这种染料的使用使我们能够证明以前未描述的缺血引起的细胞损伤事件。(C)2002 Elsevier Science B V.保留所有权利。
Animal models of ischemia are in wide use to elucidate the molecular mechanisms of brain injury that result from cardiovascular disease in humans. We have used the fluorescent, anionic dye, Fluoro-Jade, to examine cellular degeneration that occurs in association with the middle cerebral artery occlusion (MCAO) model. MCAO results in cortical infarction as well as damage to the hippocampus leading to a delayed form of death of hippocampal neurons. We examined brain sections at 6 h, 12 h, 1, 4, 7, 14 and 21 days after injury. Fluoro-Jade labeling of the striatum was seen over a protracted time-course, with degeneration beginning by 6 h after injury. Neuronal degeneration in the hippocampus, in contrast, occurs between 12 h and 7 days after injury with neuronal death reaching a peak at 4 days. GFAP/Fluoro-Jade double labeling revealed that the Fluoro-Jade positive staining at late time-points in the striatum included astrocytic cells. Together, the results show Fluoro-Jade to be a useful marker of cellular degeneration following ischemic injury. Further, the use of this dye has enabled us to demonstrate previously undescribed events of cellular injury resulting from ischemia. (C) 2002 Elsevier Science B V. All rights reserved.