Phylogeny of macrophagous leeches (Hirudinea, Clitellata) based on molecular data and evaluation of the barcoding locus
Phylogeny of macrophagous leeches (Hirudinea, Clitellata) based on molecular data and evaluation of the barcoding locus
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DOI：
10.1111/j.1463-6409.2010.00465.x
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发表时间：
2011-03-01
影响因子：
2.5
通讯作者：
Siddall, Mark E.
中科院分区：
文献类型：
--
作者：
Oceguera-Figueroa, Alejandro;Phillips, Anna J.;Siddall, Mark E.
利用线粒体标记（COI和12SrDNA）和核标记（28SrDNA和18SrDNA）对水蛭的系统发育关系进行了研究。本研究分析的完整数据集包括49个终端和5540个对齐字符。系统发育分析进行了两个最优标准：最大简约和最大似然。两个目前公认的家庭（即Erpobdellidae和Salifidae）的单系性得到证实和支持。首次对胃蛭总科的系统发育位置进行了研究，发现它是柳蛭科的姐妹科，并在Erpobdelliformes内嵌套良好。通过连续的约束分析，对以前公认的分类安排进行了评价和抛弃。形态学与生殖发育的相关性在柳蛭科中显著，但在Erpobdellidae中不显著。COI的变异性，条形码位点，进行了检查，导致识别的入侵Barbronia weberi在墨西哥，哥斯达黎加，德国，南非和台湾的物种。
The phylogenetic relationships of macrophagous leech species are studied using two mitochondrial [cytochrome c oxidase subunit I (COI) and 12S rDNA] and two nuclear (28S rDNA and 18S rDNA) markers. The complete dataset analysed in this study included 49 terminals and 5540 aligned characters. Phylogenetic analyses were performed under two optimality criteria: Maximum Parsimony and Maximum Likelihood. The monophyly of the two currently recognized families (i.e. Erpobdellidae and Salifidae) is confirmed and well supported. The phylogenetic position of Gastrostomobdellidea is studied for the first time and found to be sister to family Salifidae nested well within Erpobdelliformes. Previously recognized taxonomic arrangements were evaluated and discarded through successive constraint analyses. Correlation between morphology and phylogeny was notable in Salifidae but not in Erpobdellidae. Variability of COI, the barcoding locus, was examined across species leading to the recognition of the invasive Barbronia weberi in Mexico, Costa Rica, Germany, South Africa and Taiwan.