Immunocytochemical localization of synaptic vesicle-specific protein in lewy body-containing neurons in Parkinson's disease
Immunocytochemical localization of synaptic vesicle-specific protein in lewy body-containing neurons in Parkinson's disease
复制标题
帕金森病路易体神经元突触小泡特异性蛋白的免疫细胞化学定位
DOI：
10.1016/0304-3940(92)90923-u
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发表时间：
1992
影响因子：
2.5
通讯作者：
F. Ikuta
中科院分区：
文献类型：
--
作者：
K. Wakabayashi;H. Takahashi;K. Obata;F. Ikuta
我们进行了脑干和交感神经节的5例帕金森病尸检患者的免疫细胞化学检查，使用单克隆抗体对微管相关蛋白2（MAP 2）和突触囊泡特异性38 kDa蛋白（SVP 38）。神经细胞胞体中的路易体（LB）通常对MAP 2呈免疫反应性。神经细胞胞体中的SVP 38的免疫反应性在LB的外周部分很少被证明。另一方面，SVP 38免疫反应（IR）LB的神经细胞过程中经常发现脑干和交感神经节，大大超过MAP 2-IR LB的神经细胞过程。这些发现表明，SVP 38储存在含有LB的神经细胞过程中，并表明这些过程中的大多数是轴突。
We carried out an immunocytochemical examination of the brainstem and sympathetic ganglia of 5 autopsied patients with Parkinson's disease, using monoclonal antibodies against microtubule-associated protein 2 (MAP2) and synaptic vesicle-specific 38 kDa protein (SVP38). Lewy bodies (LBs) in the nerve cell somata were often immunoreactive for MAP2. Immunoreactivity of SVP38 in the somata of nerve cells was rarely demonstrated in the peripheral portion of the LBs. On the other hand, SVP38-immunoreactive (IR) LB-containing nerve cell processes were frequently found in the brainstem and sympathetic ganglia, considerably outnumbering MAP2-IR LB-containing nerve cell processes. These findings suggested that SVP38 is stored in LB-containing nerve cell processes and indicate that the majority of these processes are axons.