17-β-estradiol regulates expression of genes that function in macrophage activation and cholesterol homeostasis
17-β-estradiol regulates expression of genes that function in macrophage activation and cholesterol homeostasis
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DOI：
10.1016/s0960-0760(02)00065-1
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发表时间：
2002-07-01
影响因子：
4.1
通讯作者：
Wray, S
中科院分区：
文献类型：
--
作者：
Kramer, PR;Wray, S
巨噬细胞活化和胆固醇加工可受雌激素浓度变化的影响。然而，关于调节这种雌激素效应的基因和机制的信息却很少。在原代单核细胞衍生的巨噬细胞中，我们检测到雌激素受体β（ER β）的转录本和蛋白质。用cDNA阵列和半定量RT-PCR从巨噬细胞中分离RNA，确定受雌激素调节的基因，巨噬细胞在含有（5-10）× 10（-9）M 17-β-雌二醇的无血清培养基中培养，随后剥夺雌激素24 μ l。数据表明，雌激素戒断后，白细胞介素1受体拮抗剂（IL-1 ra）、β 2-微球蛋白、膜联蛋白XI和LXR α受体的转录水平显著升高，Ly-GDI转录水平显著降低：数据与雌激素耗竭调节巨噬细胞炎症和生化过程一致。在存在或不存在雌激素的情况下用佛波醇12-肉豆蔻酸酯-13-乙酸酯处理THP-1细胞表明分化成巨噬细胞样细胞类型是产生雌激素应答的先决条件。另外，实验采用放线菌酮处理。阻止新生蛋白质合成的病毒表明雌激素戒断通过不同的途径影响LXR α和IL-1 ra的转录水平。(C)2002爱思唯尔科技有限公司版权所有。
Macrophage activation and cholesterol processing can be affected by changes in estrogen concentrations. However, there is a paucity of information about the genes and mechanisms regulating this estrogen effect. In primary monocyte-derived macrophages we detected transcript and protein for estrogen receptor beta (ERbeta). Determination of genes regulated by estrogen was completed using cDNA arrays and semiquantitative RT-PCR on RNA isolated from macrophages cultured in serum free media containing (5-10) x 10(-9) M 17-beta-estradiol and subsequently deprived of estrogen for a 24 It period. The data indicate that the transcript levels of interleukin 1 receptor antagonist (IL-1ra), beta 2-microglobulin, annexin XI and the LXRalpha receptor significantly increased and that Ly-GDI transcript levels significantly decreased after estrogen withdrawal: data congruent with estrogen depletion regulating macrophage inflammatory and biochemical processes. Treatment of THP-1 cells with phorbol 12-myristate- 13-acetate in the presence or absence of estrogen indicate that differentiation to a macrophage-like cell type was a prerequisite for production of the estrogen response. In addition, experiments using cycloheximide treatment. that blocks nascent protein synthesis. indicated that estrogen withdrawal affected the transcript levels of LXRalpha and IL-1ra through dissimilar pathways. (C) 2002 Elsevier Science Ltd. All rights reserved.