Efficient Capture and T2 Magnetic Resonance Assay of Candida albicans with Inorganic Nanoparticles: Role of Nanoparticle Surface Charge and Fungal Cell Wall
Efficient Capture and T2 Magnetic Resonance Assay of Candida albicans with Inorganic Nanoparticles: Role of Nanoparticle Surface Charge and Fungal Cell Wall
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用无机纳米颗粒有效捕获白色念珠菌并进行 T2 磁共振分析：纳米颗粒表面电荷和真菌细胞壁的作用
DOI：
10.1021/acsbiomaterials.9b00069
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发表时间：
2019
影响因子：
--
通讯作者：
Wang Yilong
中科院分区：
文献类型：
--
作者：
Tian Wei;Li Fan;Wu Shengming;Li Gen;Fan Lieying;Qu Xue;Jia Xinming;Wang Yilong
通过简单易行的方法早期检测真菌是可取的。从体液样品中分离真菌在有效检测中发挥着重要作用。过去，凝集素之一的刀豆球蛋白 A (conA) 通过酵母细胞壁的表面成分与白色念珠菌相互作用。开发一种简便的方法，具有强大的结合亲和力，并能有效捕获除 conA 之外的酵母，可能会为 C 的三明治式测定带来潜力。白色念珠菌。在这项研究中，研究了静电相互作用介导的酵母捕获与 conA 介导的结合相比的可行性。此外，有效分离 C 的最佳参数。研究了白色念珠菌表面带电纳米粒子，并通过封闭实验探索了通过酵母细胞壁表面静电相互作用结合位点的机制。此外，发现酵母菌株仅通过带正电的纳米颗粒均匀地相互作用，并且捕获的酵母可以通过 FITC-conA 荧光染色和 T2 磁共振测定进行分析。因此，该策略建立了一种快速高效的真菌分离和分析方法。
The early detection of fungi through a facile and straightforward method is desirable. The isolation of fungi from a body fluid sample plays a major role in effective detection. In the past, concanavalinA (conA), one of the lectins, interacted withCandida albicansthrough the surface component of the yeast cell wall. The development of a facile method with a robust binding affinity and an efficient capture of the yeast in addition to conA could pose a potential for the sandwich-like assay ofC. albicans. In this study, the feasibility of an electrostatic interaction-mediated yeast capture was investigated as compared to conA-mediated binding. Also, the optimal parameters for the efficient isolation ofC. albicansby surface-charged nanoparticles were studied, and the mechanism of the binding site through the electrostatic interaction on the surface of the yeast cell wall was explored by a blocking experiment. Furthermore, the yeast strains were found to interact uniformly only via the positively charged nanoparticles, and the captured yeast could be analyzed by FITC-conA fluorescence staining and T2 magnetic resonance assay. Thus, this strategy established a rapid and highly efficient method for the isolation of fungi and analysis.