Metabolism of DMSP, DMS and DMSO by the cultivable bacterial community associated with the DMSP-producing dinoflagellate Scrippsiella trochoidea
Metabolism of DMSP, DMS and DMSO by the cultivable bacterial community associated with the DMSP-producing dinoflagellate Scrippsiella trochoidea
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DOI：
10.1007/s10533-012-9702-7
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发表时间：
2012-09-01
期刊：
影响因子：
4
通讯作者：
Green, David H.
中科院分区：
文献类型：
--
作者：
Hatton, Angela D.;Shenoy, Damodar M.;Green, David H.
培养和鉴定了与产二甲基磺基丙酸盐（DMSP）浮游植物锥状斯氏藻（Scrippsiella trochoidea）相关的细菌种类，目的是确定它们代谢DMSP、二甲基硫醚（DMS）和二甲基亚砜（DMSO）的能力。结果表明，在可培养的细菌中，只有α-变形菌能够从DMSP产生DMS。DMSP的浓度被证明会影响所产生的DMS的量。较低的DMSP浓度（1.5 μ mol dm（-3））被完全同化，而较高的浓度（10 μ mol dm（-3））导致产生的DMS量增加。与代谢DMSP的有限细菌组相比，近似70%的细菌分离物能够“消耗”DMS。然而，98-100%的DMS被除去，作为DMSO。值得注意的是，这些细菌中的一些只会在葡萄糖存在下氧化DMS，包括γ-变形菌门和拟杆菌门的成员。从这项研究中的观察，再加上已发表的现场数据，确定DMS氧化为DMSO作为DMS的主要转化途径，我们推测，DMS和DMSP在该领域的命运是紧密耦合到浮游植物产生的可用碳。
Bacterial species associated with the dimethylsulfoniopropionate (DMSP)-producing phytoplankton Scrippsiella trochoidea were cultured and identified, with the aim of establishing their ability to metabolise DMSP, dimethylsulfide (DMS) and dimethylsulfoxide (DMSO). Results demonstrate that of the cultivable bacteria only alpha-Proteobacteria were capable of producing DMS from DMSP. The concentration of DMSP was shown to affect the amount of DMS produced. Lower DMSP concentrations (1.5 mu mol dm(-3)) were completely assimilated, whereas higher concentrations (10 mu mol dm(-3)) resulted in increasing amounts of DMS being produced. By contrast to the restricted set of bacteria that metabolised DMSP,similar to 70% of the bacterial isolates were able to 'consume' DMS. However, 98-100% of the DMS removed was accounted for as DMSO. Notably, a number of these bacteria would only oxidise DMS in the presence of glucose, including members of the gamma-Proteobacteria and Bacteroidetes. The observations from this study, coupled with published field data, identify DMS oxidation to DMSO as a major transformation pathway for DMS, and we speculate that the fate of DMS and DMSP in the field are tightly coupled to the available carbon produced by phytoplankton.