A conserved oomycete CRN effector targets tomato TCP14-2 to enhance virulence.
A conserved oomycete CRN effector targets tomato TCP14-2 to enhance virulence.
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DOI：
10.1094/mpmi-06-20-0172-r
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发表时间：
2020-12
期刊：
影响因子：
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通讯作者：
R. Stam;Graham B. Motion;Victor Martinez-Heredia;P. Boevink;E. Huitema
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文献类型：
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作者：
R. Stam;Graham B. Motion;Victor Martinez-Heredia;P. Boevink;E. Huitema
疫霉属在感染期间分泌大量效应分子以帮助宿主定殖。CRN蛋白家族形成了广泛的候选效应子库，其在宿主细胞核中积累以干扰免疫所需的过程。在这里，我们表明来自辣椒疫霉的CRN12_997结合TCP转录因子SlTCP 14 -2，以抑制其对疫霉的免疫相关活性。共免疫沉淀和分裂-YFP研究证实了体内特异性CRN12_997-SlTCP 14 -2相互作用。CRN12_997的共表达特异性地抵消了TCP 14增强的免疫表型，表明CRN介导的SlTCP 14 -2功能的扰动。我们表明，SlTCP 14 -2与核染色质相关，CRN12_997减少了SlTCP 14 -2 DNA结合。总的来说，我们的数据支持SlTCP 14 -2与染色质缔合以增强免疫力的模型。CRN12_997和SlTCP 14 -2之间的相互作用降低了免疫调节剂的DNA结合。我们提出由CRN 12 -997引起的SlTCP 14 -2染色质亲和力的调节增强了对辣椒疫霉的易感性。
Phytophthora spp. secrete vast arrays of effector molecules during infection to aid in host colonization. The CRN protein family forms an extensive repertoire of candidate effectors that accumulate in the host nucleus to perturb processes required for immunity. Here, we show that CRN12_997 from P. capsici binds a TCP transcription factor, SlTCP14-2, to inhibit its immunity-associated activity against Phytophthora. Co-immuno-precipitation and split-YFP studies confirm a specific CRN12_997-SlTCP14-2 interaction in vivo. Co-expression of CRN12_997 specifically counteracts the TCP14-enhanced immunity phenotype, suggesting CRN mediated perturbation of SlTCP14-2 function. We show that SlTCP14-2 associates with nuclear chromatin and that CRN12_997 diminishes SlTCP14-2 DNA-binding. Collectively, our data support a model in which SlTCP14-2 associates with chromatin to enhance immunity. The interaction between CRN12_997 and SlTCP14-2 reduces DNA-binding of the immune regulator. We propose that the modulation of SlTCP14-2 chromatin affinity, caused by CRN12-997, enhances susceptibility to Phytophthora capsici.