Development and Evaluation of Intron and Insertion–Deletion Markers for Gossypium barbadense
Development and Evaluation of Intron and Insertion–Deletion Markers for Gossypium barbadense
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DOI：
10.1007/s11105-011-0369-3
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发表时间：
2012-06
影响因子：
2.1
通讯作者：
Xiaqing Wang;G. Ren;Ximei Li;J. Tu;Zhongxu Lin;Xianlong Zhang
中科院分区：
文献类型：
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作者：
Xiaqing Wang;G. Ren;Ximei Li;J. Tu;Zhongxu Lin;Xianlong Zhang
从核苷酸数据库中选取588个棉巴氏二次测序序列（CDSs）进行标记开发。选择后，利用125个CDSs设计126个标记，包括39个内含子多态性（GbIPs）和87个插入-删除多态性（GbIDPs）。通过对包括56G在内的66份四倍体棉花材料的遗传多样性分析，对这些标记进行了评价。barbadenseaccations和10ssypium hirsutumaccations。多态性信息含量的扩增效率分别为0.560和0.489，有效多重比(E)的扩增效率分别为0.744和0.690，数据性质定性的扩增效率分别为0.653和0.438，有效标记指数的扩增效率分别为0.272和0.148。主坐标分析表明，两者之间存在着深刻的差异。hirsutumandG。barbadenseaccessions。此外，大多数g。中国对新疆的割据与外国和其他中国割据有着明显的区别。barbadenseaccessions。126个标记丰富遗传图谱的能力进行了评估，在9条染色体上定位了16个多态性位点，其中6个位点在A亚基因组上，10个位点在D亚基因组上。GbIPs和GbIDPs引物的定位效率分别为15.38%和11.49%。该研究很好地证明了GbIPs和GbIDPs可以成功地应用于遗传多样性分析和遗传图谱的构建。
A total of 588Gossypium barbadensecoding sequences (CDSs) from nucleotide databases were selected for marker development. After selection, 125 CDSs were used to design 126 markers, including 39 intron polymorphisms (GbIPs) and 87 insertion–deletion polymorphisms (GbIDPs). These markers were evaluated by analyzing the genetic diversity of 66 tetraploid cotton accessions including 56G. barbadenseaccessions and 10Gossypium hirsutumaccessions. The amplification efficiencies of the GbIPs and GbIDPs were 0.560 and 0.489 for polymorphism information content, 0.744 and 0.690 for effective multiplex ratio (E), 0.653 and 0.438 for qualitative of nature of data, and 0.272 and 0.148 for effective marker index. Principal coordinate analysis showed profound differences betweenG. hirsutumandG. barbadenseaccessions. In addition, most of theG. barbadenseaccessions of Xinjiang, China were clearly different from foreign and other ChineseG. barbadenseaccessions. The 126 markers were also evaluated for their ability to enrich genetic maps, and 16 polymorphic loci were mapped on nine chromosomes with six loci on A subgenome and 10 loci on D subgenome. The mapping efficiencies of GbIPs and GbIDPs primers were 15.38% and 11.49%, respectively. This study well proves that GbIPs and GbIDPs can be successfully applied to the analysis of genetic diversity and construction of genetic maps.