The effect of different combinations of antibiotic cocktails on mice and selection of animal models for further microbiota research
The effect of different combinations of antibiotic cocktails on mice and selection of animal models for further microbiota research
复制标题
抗生素混合物的不同组合对小鼠的影响以及为进一步微生物群研究选择动物模型。
DOI：
10.1007/s00253-021-11131-2
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发表时间：
2021-01-29
影响因子：
5
通讯作者：
Nie, Yu-qiang
中科院分区：
文献类型：
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作者：
Xu, Jing;Xu, Hao-ming;Nie, Yu-qiang
肠道微生物群与宿主的健康和疾病密切相关。然而，目前还没有合适的动物模型来探索其功能。我们分析了3种不同的抗生素鸡尾酒（ABx）通过两种给药途径对小鼠肠道菌群组成以及一般生理和代谢指标的影响。高通量16S rRNA测序表明，ABx治疗改变了肠道微生物群的结构，也引起了结肠的轻度炎症。此外，与直接经口灌胃相比，随意给予抗生素更有效地消耗肠道微生物群，尤其是3ABx。ABx治疗还对肾和肝功能产生显著影响，如肌酐、尿素、总甘油三酯和总胆固醇的血清水平改变所示。最后，斯皮尔曼的相关性分析表明，ABx干预产生的优势细菌属，包括乳杆菌属、罗斯拜瑞氏菌属和嗜酸单胞菌属，与肾功能指标呈负相关。总之，不同的抗生素组合和干预措施会耗尽肠道微生物群并诱导宿主的生理变化。我们的研究结果为开发用于研究肠道微生物群的适应性动物模型提供了基础。
The gut microbiota is closely related to host health and disease. However, there are no suitable animal models available at present for exploring its functions. We analyzed the effect of 3 different antibiotic cocktails (ABx) via two administration routes on the composition of murine gut microbiota, as well as on the general physiological and metabolic indices. High-throughput 16S rRNA sequencing showed that ABx treatment altered the gut microbiota community structure, and also caused low-degree inflammation in the colon. In addition, ad libitum administration of antibiotics depleted the gut microbiota more effectively compared to direct oral gavage, especially with 3ABx. The ABx treatment also had a significant impact on renal and liver functions, as indicated by the altered serum levels of creatinine, urea, total triglycerides, and total cholesterol. Finally, Spearman's correlation analysis showed that the predominant bacterial genera resulting from ABx intervention, including Lactobacillus, Roseburia, and Candidatus-Saccharimonas, were negatively correlated with renal function indices. Taken together, different antibiotic combinations and interventions deplete the gut microbiota and induce physiological changes in the host. Our findings provide the basis for developing an adaptive animal model for studying gut microbiota.