Henriciella litoralis sp. nov., isolated from a tidal flat, transfer of Maribaculum marinum Lai et al. 2009 to the genus Henriciella as Henriciella aquimarina nom. nov. and emended description of the genus Henriciella.
Henriciella litoralis sp. nov., isolated from a tidal flat, transfer of Maribaculum marinum Lai et al. 2009 to the genus Henriciella as Henriciella aquimarina nom. nov. and emended description of the genus Henriciella.
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DOI：
10.1099/ijs.0.020396-0
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发表时间：
2011-04
影响因子：
2.8
通讯作者：
S. H. Lee;J. Shim;J. Kim;Hyung-Kyoon Choi;C. Jeon
中科院分区：
文献类型：
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作者：
S. H. Lee;J. Shim;J. Kim;Hyung-Kyoon Choi;C. Jeon
从韩国黄海滩涂分离到一株革兰氏染色阴性、需氧严格的细菌，命名为SD10(T)。细胞是不形成孢子的杆状细胞，表现出过氧化氢酶和氧化酶阳性反应。菌株SD10(T)在15-40℃(最适25-30℃)、pH 6.0-9.0(最适pH 6.5-8.5)和1-10%(w/v)的氯化钠存在下生长。菌株SD10(T)含有主要的异戊二烯对苯二酚-10(Q-10)，主要脂肪酸为C(18：1)ω7c(39.3%)、C(16：0)(20.2%)、C(17：0)(8.9%)和C(17：1)ω6c(8.1%)。细胞内的极性脂被鉴定为磷脂酰甘油、单甘油二甘酯、葡萄糖醛酸吡喃二甘油酯和两种未知的糖脂。基因组DNA中G+C含量为55.2mol%。根据16S rRNA基因序列的相似性，该菌株与海洋亨利希氏菌Iso4(T)和马里巴氏菌P38(T)的亲缘关系最近，相似性分别为97.8%和97.0%。菌株SD10(T)与海洋梭子藻Iso4(T)的DNA-DNA同源性为12.0±3.2%。基于16S rRNA基因序列的系统发育分析表明，海洋分枝杆菌P38(T)和海洋分枝杆菌Iso4(T)是一个单系统类群，它们的16S rRNA基因序列相似性为98.1%。海洋微囊藻Iso4(T)与海洋微囊藻24711(T)之间的杂交率为22.9±2.7%，表明这两个菌株属于不同的种。根据化学分类数据和分子生物学特性，我们认为SD10(T)菌株是一新种，命名为Henriciella litoralis sp.11月是提议的。模式菌株为SD10(T)(=KAcc 13700(T)=Dsm 22014(T))。此外，我们还建议将Maribaculum marum Lai等人。2009将其命名为Henriciella Aquimarina nom.11月(模式菌株P38(T)=CCTCC AB 208227(T)=1MG 24711(T)=MCCC1A01086(T))，并对该属进行了补充描述。
A Gram-staining-negative, strictly aerobic bacterium, designated strain SD10(T), was isolated from a tidal flat of the Yellow Sea, South Korea. Cells were non-spore-forming rods that showed catalase- and oxidase-positive reactions. Growth of strain SD10(T) was observed at 15-40 °C (optimum, 25-30 °C), at pH 6.0-9.0 (optimum, pH 6.5-8.5) and in the presence of 1-10 % (w/v) NaCl. Strain SD10(T) contained ubiquinone-10 (Q-10) as a major isoprenoid quinone and C(18 : 1)ω7c (39.3 %), C(16 : 0) (20.2 %), C(17 : 0) (8.9 %) and C(17 : 1)ω6c (8.1 %) as major fatty acids. The cellular polar lipids were identified as phosphatidylglycerol, monoglycosyldiglyceride, glucuronopyranosyldiglyceride and two unidentified glycolipids. The G+C content of the genomic DNA was 55.2 mol%. Based on 16S rRNA gene sequence similarities, the strain was most closely related to Henriciella marina Iso4(T) and Maribaculum marinum P38(T), with similarities of 97.8 and 97.0 %, respectively. The DNA-DNA relatedness between strain SD10(T) and H. marina Iso4(T) was 12.0±3.2 %. A phylogenetic analysis based on 16S rRNA gene sequences showed that M. marinum P38(T) and H. marina Iso4(T) formed a monophyletic cluster and that their 16S rRNA gene sequence similarity was 98.1 %. DNA-DNA hybridization between H. marina Iso4(T) and M. marinum LMG 24711(T) was 22.9±2.7 %, indicating that the two strains belong to separate species. On the basis of chemotaxonomic data and molecular properties, we propose that strain SD10(T) represents a novel species of the genus Henriciella, for which the name Henriciella litoralis sp. nov. is proposed. The type strain is SD10(T) ( = KACC 13700(T) = DSM 22014(T)). In addition, we propose to transfer Maribaculum marinum Lai et al. 2009 to the genus Henriciella as Henriciella aquimarina nom. nov. (type strain P38(T) = CCTCC AB 208227(T) = LMG 24711(T) = MCCC 1A01086(T)), and we present an emended description of the genus Henriciella.