Analysis of Brain Structure and Neural Organization in Dystrophin-Deficient Model Mice with Magnetic Resonance Imaging at 7 T
Analysis of Brain Structure and Neural Organization in Dystrophin-Deficient Model Mice with Magnetic Resonance Imaging at 7 T
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DOI：
10.2174/18744400-v15-e2202040
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发表时间：
2022-03
期刊：
影响因子：
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通讯作者：
Mitsuki Rikitake;J. Hata;Mayu Iida;Fumiko Seki;Rina Ito;Yuji Komaki;Chihoko Yamada;D. Yoshimaru;H. Okano;T. Shirakawa
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文献类型：
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作者：
Mitsuki Rikitake;J. Hata;Mayu Iida;Fumiko Seki;Rina Ito;Yuji Komaki;Chihoko Yamada;D. Yoshimaru;H. Okano;T. Shirakawa
肌营养不良蛋白增强肌肉细胞;然而，在肌营养不良症中，肌营养不良蛋白由于糖链异常而缺乏。这种异常发生在骨骼肌和脑组织中。本研究旨在非侵入性地分析肌肉萎缩症患者大脑的神经组织。我们使用了一个小鼠模型的肌营养不良症，以研究是否可以通过7 T磁共振成像评估的脑结构和神经退行性变的肌营养不良蛋白缺乏。C57 BL/10-mdx（X染色体连锁肌营养不良）小鼠用作营养不良小鼠模型，健康小鼠用作对照。心室扩大是肌营养不良蛋白缺乏患者最常见的脑畸形之一。因此，我们使用横向弛豫加权成像检查C57 BL/10-mdx是否观察到心室扩大。使用弥散张量图像和神经突方向弥散和密度成像的弥散MRI进行脑实质分析。实质变性进行了评估的方向扩散，神经纤维扩散，和树突状散射密度。对于通过T2 WI分析的脑室体积，mdx小鼠的平均大小是对照小鼠的1.5倍。在脑实质中，观察到指示神经纤维方向紊乱的参数和白色物质区域的树突散射密度存在显著差异（p < 0.05）。我们的研究结果表明，由于抗肌萎缩蛋白缺乏症的脑结构的变化，可以详细评估，而不破坏组织相结合的扩散张量图像和神经突的方向分散和密度成像分析。
Dystrophin strengthens muscle cells; however, in muscular dystrophy, dystrophin is deficient due to an abnormal sugar chain. This abnormality occurs in skeletal muscle and in brain tissue. This study aimed to non-invasively analyze the neural organization of the brain in muscular dystrophy. We used a mouse model of muscular dystrophy to study whether changes in brain structure and neurodegeneration following dystrophin deficiency can be assessed by 7T magnetic resonance imaging. C57BL/10-mdx (X chromosome-linked muscular dystrophy) mice were used as the dystrophic mouse model and healthy mice were used as controls. Ventricular enlargement is one of the most common brain malformations in dystrophin-deficient patients. Therefore, we examined whether ventricular enlargement was observed in C57BL/10-mdx using transverse-relaxation weighted images. Brain parenchyma analysis was performed using diffusion MRI with diffusion tensor images and neurite orientation dispersion and density imaging. Parenchymal degeneration was assessed in terms of directional diffusion, nerve fiber diffusion, and dendritic scattering density. For the volume of brain ventricles analyzed by T2WI, the average size was 1.5 times larger in mdx mice compared to control mice. In the brain parenchyma, a significant difference (p < 0.05) was observed in parameters indicating disturbances in the direction of nerve fibers and dendritic scattering density in the white matter region. Our results show that changes in brain structure due to dystrophin deficiency can be assessed in detail without tissue destruction by combining diffusion tensor images and neurite orientation dispersion and density imaging analyses.