Sample handling of gastric tissue and O-glycan alterations in paired gastric cancer and non-tumorigenic tissues.
Sample handling of gastric tissue and O-glycan alterations in paired gastric cancer and non-tumorigenic tissues.
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DOI：
10.1038/s41598-017-18299-6
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发表时间：
2018-01-10
影响因子：
4.6
通讯作者：
Karlsson NG
中科院分区：
文献类型：
--
作者：
Adamczyk B;Jin C;Polom K;Muñoz P;Rojas-Macias MA;Zeeberg D;Borén M;Roviello F;Karlsson NG
样本采集、处理和储存是确保最大限度保存样本的最关键步骤。分析前的变异性可能会影响结果，因为组织切除后或长期储存期间蛋白质特征会迅速改变。因此，我们从糖组学的角度评估了目前最先进的生物库保存方法，并分析了胃癌组织中发生的O-聚糖改变。配对的肿瘤和相邻的正常组织样本来自6名接受胃癌手术的患者。将收集的样品（n = 24）速冻或热稳定，然后均质化。从提取的糖蛋白中释放聚糖，并通过LC-MS/MS进行分析。总共使用83种O-聚糖的相对丰度和17种衍生的结构特征进行比较。速冻和热稳定样品之间的变量没有统计学显著差异，这表明两种保存方法具有可比性。这些数据还显示了正常组织和癌组织之间的显着变化。除了从癌症区域中的高唾液酸化向正常区域中的血型ABO的转变之外，我们还检测到LacdiNAc表位（N，N '-二乙酰基乳糖胺）在癌症样品中显著降低。本文提出的O-聚糖改变可能为胃癌的检测和预后提供预测能力。
Sample collection, handling and storage are the most critical steps for ensuring the highest preservation of specimens. Pre-analytical variability can influence the results as protein signatures alter rapidly after tissue excision or during long-term storage. Hence, we evaluated current state-of-the-art biobank preservation methods from a glycomics perspective and analyzed O-glycan alterations occurring in the gastric cancer tissues. Paired tumor and adjacent normal tissue samples were obtained from six patients undergoing gastric cancer surgery. Collected samples (n = 24) were either snap-frozen or heat stabilized and then homogenized. Glycans were released from extracted glycoproteins and analyzed by LC-MS/MS. In total, the relative abundance of 83 O-glycans and 17 derived structural features were used for comparison. There was no statistically significant difference found in variables between snap frozen and heat-stabilized samples, which indicated the two preservation methods were comparable. The data also showed significant changes between normal and cancerous tissue. In addition to a shift from high sialylation in the cancer area towards blood group ABO in the normal area, we also detected that the LacdiNAc epitope (N,N’-diacetyllactosamine) was significantly decreased in cancer samples. The O-glycan alterations that are presented here may provide predictive power for the detection and prognosis of gastric cancer.
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影响因子：
3.7
作者：
Hossler P;Mulukutla BC;Hu WS
通讯作者：
Hu WS
影响因子：
14.8
作者：
Jensen, Pia H.;Karlsson, Niclas G.;Packer, Nicolle H.
通讯作者：
Packer, Nicolle H.
影响因子：
3
作者：
Ahmed, Md Mahiuddin;Gardiner, Katheleen J.
通讯作者：
Gardiner, Katheleen J.
DOI：
10.1016/j.bbapap.2013.04.018
发表时间：
2014-01-01
影响因子：
3.2
作者：
Campbell, Matthew P.;Nguyen-Khuong, Terry;Karlsson, Niclas G.
通讯作者：
Karlsson, Niclas G.
DOI：
10.1007/978-1-4939-6493-2_8
发表时间：
2017-01-01
期刊：
HIGH-THROUGHPUT GLYCOMICS AND GLYCOPROTEOMICS: METHODS AND PROTOCOLS
影响因子：
--
作者：
Adamczyk, Barbara;Stockmann, Henning;Rudd, Pauline M.
通讯作者：
Rudd, Pauline M.