PET imaging of hepatocellular carcinoma with 2-deoxy-2[18F]fluoro-D-glucose, 6-deoxy-6[18F] fluoro-D-glucose, [1-11C]-acetate and [N-methyl-11C]-choline.
PET imaging of hepatocellular carcinoma with 2-deoxy-2[18F]fluoro-D-glucose, 6-deoxy-6[18F] fluoro-D-glucose, [1-11C]-acetate and [N-methyl-11C]-choline.
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发表时间：
2009-04
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通讯作者：
N. Salem;Y. Kuang;F. Wang;Maclennan Gt;Zhenghong Lee
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作者：
N. Salem;Y. Kuang;F. Wang;Maclennan Gt;Zhenghong Lee
目的本研究旨在研究使用现有示踪剂如2-脱氧-2[(18)F]氟-D-葡萄糖(2FDG)、6-脱氧-6[(18)F]氟-D-葡萄糖(6FDG)、[1(-11)C]乙酸盐在肝炎病毒感染诱导的土拨鼠模型上对肝细胞癌(HCC)进行正电子发射断层扫描(PET)成像的性能（乙酸盐）和[N-甲基（-11）C]胆碱（胆碱）。方法 使用不同的放射性示踪剂对 14 只患有 HCC 的土拨鼠进行成像：13 只（10 只患有 HCC，3 只对照）使用 2FDG；13 只（10 只患有 HCC，3 只对照）使用 2FDG； 4 个（3 个患有 HCC，1 个对照）使用 6FDG； 13 例（10 例患有 HCC，3 例为对照）使用醋酸盐； 4 例（2 例 HCC 患者和 2 例对照者）使用胆碱。成像实验后对土拨鼠进行安乐死，收集肝组织用于组织学分析，包括己糖激酶（HK）、葡萄糖-6-磷酸酶、乙酰辅酶A合成酶（ACAS）和胆碱激酶（CK）等酶活性，以及HCC与周围肝组织之间的差异基因表达。结果 2FDG 检测到 7/13 个肿瘤，肿瘤与肝脏的摄取比 (T/L) 为 1.36+/-0.13。其中 5 个 HCC 为中分化或低分化。与周围肝组织相比，HCC 中的 HK/葡萄糖-6-磷酸酶比值显着较高（P=0.05）。 PET 未检测到 6FDG 成像的 HCC（T/L=1.01+/-0.11）。醋酸盐检测到 16/17 个 HCC (T/L=2.02+/-0.7)。 HCC 中的 ACAS 活性显着较高（P=0.01），并且发现脂质相关基因上调。胆碱成像检测到所有 HCC（T/L=1.63+/-0.34）。 HCC 中的 CK 活性显着较高（P=0.001）。结论 高分化和一些中分化的 HCC 摄取 2FDG 的量并不比周围肝组织多，但醋酸盐摄取量增加。 6FDG PET 图像上 HCC 与周围肝组织没有对比度。尽管来自肝脏的背景信号升高，但在本研究中扫描的 HCC 中似乎可以检测到胆碱摄取。
AIM This study was designed to investigate the performance of positron emission tomography (PET) imaging for hepatocellular carcinoma (HCC) on a hepatitis viral infection-induced woodchuck model using existing tracers such as 2-deoxy-2[(18)F]fluoro-D-glucose (2FDG), 6-deoxy-6[(18)F]fluoro-D-glucose (6FDG), [1(-11)C]acetate (acetate) and [N-methyl(-11)C]choline (choline). METHODS Fourteen woodchucks with HCC were imaged with different radiotracers: 13 (10 with HCC and 3 controls) with 2FDG; 4 (3 with HCC and 1 control) with 6FDG; 13 (10 with HCC and 3 controls) with acetate; 4 (2 with HCC and 2 controls) with choline. The woodchucks were euthanized after imaging experiments and liver tissues were harvested for histology, for enzymatic activities including hexokinase (HK), glucose-6-phosphatase, acetyl-CoA synthetase (ACAS) and choline kinase (CK), and for differential gene expressions between the HCCs and the surrounding hepatic tissues. RESULTS 2FDG detected 7/13 tumors with a tumor-to-liver uptake ratio (T/L) of 1.36+/-0.13. Five of these HCCs were moderately- or poorly-differentiated. The HK/glucose-6-phosphatase ratio was significantly higher in HCCs compared to the surrounding liver tissues (P=0.05). None of the HCCs imaged with 6FDG were detected by PET (T/L=1.01+/-0.11). Acetate detected 16/17 HCCs (T/L=2.02+/-0.7). ACAS activity was significantly higher in HCCs (P=0.01) and lipids-related genes were found up-regulated. Choline imaging detected all HCCs (T/L=1.63+/-0.34). CK activity was significantly higher in HCCs (P=0.001). CONCLUSIONS Well-differentiated and some moderately-differentiated HCCs do not uptake 2FDG more than the surrounding liver tissues, but display increased acetate uptake. There is no contrast between HCCs and the surrounding liver tissues on the 6FDG PET images. Despite elevated background signal from the liver, choline uptake seems to be detectable in the HCCs scanned in this study.