Slow magic angle sample spinning: a non- or minimally invasive method for high-resolution 1H nuclear magnetic resonance (NMR) metabolic profiling.
Slow magic angle sample spinning: a non- or minimally invasive method for high-resolution 1H nuclear magnetic resonance (NMR) metabolic profiling.
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DOI：
10.1007/978-1-61737-985-7_20
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发表时间：
2011
影响因子：
--
通讯作者：
J. Hu
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文献类型：
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作者：
J. Hu
高分辨率1H魔角旋转核磁共振（NMR），使用几千赫兹或更高的样品旋转速率（即，高分辨率魔角旋转（hr-MAS））是一种用于完整组织中代谢谱分析而无需样品提取的成熟方法。hr-MAS的唯一缺点是它是侵入性的，因此不能用于非破坏性检测。最近，一种称为慢MAS的方法，使用二维NMR光谱学的概念，由于使用缓慢或超缓慢的样品旋转，已经成为完整组织（包括活动物）中非侵入性或微创代谢组学的替代方法。虽然慢MAS是一种强大的方法，但其应用受到实验挑战的阻碍。正确设计实验和选择适当的慢MAS方法都需要对操作原理有基本的了解，特别是由于分子扩散的存在而导致的谱线变窄的细节。然而，这些基本原则尚未在以前的出版物中充分披露。本章的目标是通过强调与玻璃珠和H2O组成的体模样本相关的挑战，对与慢速MAS技术相关的原理进行深入评估，其中获得了异常大的磁化率场梯度。
High-resolution1H magic angle spinning nuclear magnetic resonance (NMR), using a sample spinning rate of several kilohertz or more (i.e., high-resolution magic angle spinning (hr-MAS)), is a well-established method for metabolic profiling in intact tissues without the need for sample extraction. The only shortcoming with hr-MAS is that it is invasive and is thus unusable for non-destructive detections. Recently, a method called slow MAS, using the concept of two-dimensional NMR spectroscopy, has emerged as an alternative method for non- or minimally invasive metabolomics in intact tissues, including live animals, due to the slow or ultra-slow sample spinning used. Although slow MAS is a powerful method, its applications are hindered by experimental challenges. Correctly designing the experiment and choosing the appropriate slow MAS method both require a fundamental understanding of the operation principles, in particular the details of line narrowing due to the presence of molecular diffusion. However, these fundamental principles have not yet been fully disclosed in previous publications. The goal of this chapter is to provide an in-depth evaluation of the principles associated with slow MAS techniques by emphasizing the challenges associated with a phantom sample consisting of glass beads and H2O, where an unusually large magnetic susceptibility field gradient is obtained.