Production of the long pentraxin PTX3 in advanced atherosclerotic plaques
Production of the long pentraxin PTX3 in advanced atherosclerotic plaques
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DOI：
10.1161/01.atv.0000015595.95497.2f
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发表时间：
2002-05-01
影响因子：
8.7
通讯作者：
Hansson, GK
中科院分区：
文献类型：
--
作者：
Rolph, MS;Zimmer, S;Hansson, GK
五聚蛋白家族成员C反应蛋白（CRP）的血浆水平升高与健康和高危受试者心血管疾病风险增加相关。长五聚蛋白家族成员，五聚蛋白3（PTX 3），最近被描述。与CRP一样，PTX 3由急性炎症刺激诱导，并在急性心肌梗死患者的血液中增加。与CRP不同，它在广泛的细胞类型中表达，但不在肝细胞中表达。在这项研究中，我们研究了PTX 3在动脉粥样硬化中的表达。免疫组化染色显示晚期动脉粥样硬化病变的PTX 3强表达。相比之下，在非动脉粥样硬化性内乳动脉中未观察到PTX 3表达。通过用细胞类型和PTX 3特异性抗体染色连续切片，我们观察到PTX 3主要由巨噬细胞和内皮细胞产生。还观察到平滑肌细胞的罕见表达。我们的研究结果表明，PTX 3可能有助于动脉粥样硬化的发病机制。
Elevated plasma levels of the pentraxin protein family member C-reactive protein (CRP) are associated with increased risk of cardiovascular disease in both healthy and high-risk subjects. The long pentraxin family member, pentraxin 3 (PTX3), was recently described. Like CRP, PTX3 is induced by acute inflammatory stimuli and is increased in the blood of patients with acute myocardial infarction. Unlike CRP, it is expressed in a wide range of cell types, but not in hepatocytes. In this study, we have investigated the expression of PTX3 in atherosclerosis. Immunohistochemical staining of advanced atherosclerotic lesions revealed strong expression of PTX3. In contrast, no PTX3 expression was observed in nonatherosclerotic internal mammary arteries. By staining serial sections with cell type- and PTX3-specific antibodies, we observed that PTX3 was produced principally by macrophages and endothelial cells. Infrequent expression by smooth muscle cells was also observed. Our results suggest that PTX3 may contribute to the pathogenesis of atherosclerosis.