Morphological and proteomic characterization of midgut of the malaria vector Anopheles albimanus at early time after a blood feeding
Morphological and proteomic characterization of midgut of the malaria vector Anopheles albimanus at early time after a blood feeding
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DOI：
10.1016/j.jprot.2014.07.037
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发表时间：
2014-12-05
影响因子：
3.3
通讯作者：
Hernandez-Hernandez, F. C.
中科院分区：
文献类型：
--
作者：
Cazares-Raga, F. E.;Chavez-Munguia, B.;Hernandez-Hernandez, F. C.
按蚊的中肠是疟疾的病原体--疟原虫--的入口。当蚊子以受寄生虫感染的宿主为食时，疟原虫到达中肠，必须面对消化酶和先天免疫反应，并穿过由蚊子中肠上皮细胞分泌的厚厚的细胞外基质(PM)。然后，为了继续它的发展，寄生虫必须到达唾液腺才能传播给脊椎动物宿主。在这里，我们报告了白纹伊蚊在进食血液后中肠的形态和生化变化。采血前，中肠上皮细胞内含有大量电子致密泡，分布于中央至顶端。这些小泡在血餐后被分泌到中肠的管腔一侧。在血液摄入的早期，PM形成在微绒毛附近，作为一种颗粒状的无定形物质，在它固结后形成高度有序的纤维结构，由多层电子致密和电子透明区域组成。对食糖和供血中肠的蛋白质组学比较分析表明，在摄血后，有几种分子会改变它们的丰度；这些分子包括先天性免疫、细胞骨架、应激反应、信号以及消化、解毒和代谢酶。生物学意义在蚊子的中肠中，有许多同时发生的过程，包括消化、先天免疫活动、细胞骨架修饰、构建营养周围基质和激素产生等。在进食过程中，机械力非常强，上皮细胞和肌肉细胞必须抵抗压力，修改肌动蛋白细胞骨架，并通过信号来协调细胞内的反应。中肠内容物中的微生物繁殖并与上皮细胞相互作用，触发先天免疫反应。当感染病原体出现在血餐中时，它们必须穿过营养周围基质，即由上皮细胞分泌产物形成的包膜，并避开免疫系统，以到达上皮细胞，继续向唾液腺前进，为传播给新宿主做准备。在所有这些过程中，蚊子的蛋白质被修饰以应对机械和生物挑战，这项工作的目的是研究这些变化。本文是2013年坎昆一期题为：蛋白质组学、质谱学和多肽的特刊的一部分。客座编辑：Cesar Lopez-Camarillo，Victoria Pando-Robles和Bronwyn Jane Barlda(C)2014爱思唯尔B.V.保留所有权利。
The midgut of anopheline mosquito is the entry of Plasmodium, the causative agent of malaria. When the mosquito feeds on parasite infected host, Plasmodium parasites reach the midgut and must confront digestive enzymes, the innate immune response and go across the peritrophic matrix (PM), a thick extracellular sheath secreted by the mosquito midgut epithelial cells. Then, to continue its development, the parasite must reach the salivary glands to achieve transmission to a vertebrate host.We report here the morphological and biochemical descriptions of the midgut changes after a blood meal in Anopheles albimanus. Before blood feeding, midgut epithelial cells contained numerous electrondense vesicles distributed in the central to apical side. These vesicles were secreted to the luminal side of the midgut after a blood meal. At early times after blood ingest, the PM is formed near microvilli as a granulous amorphous material and after it consolidates forming a highly organized fibrillar structure, constituted by layers of electrondense and electronlucent regions. Proteomic comparative analysis of sugar and blood fed midguts showed several molecules that modify their abundance after blood intake; these include innate immunity, cytoskeletal, stress response, signaling, and digestive, detoxifying and metabolism enzymes.Biological significanceIn the midgut of mosquitoes during bloodfeeding, many simultaneous processes occur, including digestion, innate immune activities, cytoskeleton modifications, construction of a peritrophic matrix and hormone Production, between others. Mechanical forces are very intense during bloodfeeding and epithelial and muscular cells must resist the stress, modifying the actin cytoskeleton and coordinating intracellular responses by signaling. Microorganisms present in midgut contents reproduce and interact with epithelial cells triggering innate immune response. When infectious agents are present in the blood meal they must traverse the peritrophic matrix, an envelope formed from secretion products of epithelial cells, and evade the immune system in order to reach the epithelium and continue their journey towards salivary glands, in preparation for the transmission to the new hosts. During all these processes, proteins of mosquitoes are modified in order to deal with mechanical and biological challenges, and the aim of this work is to study these changes.This article is part of a Special Issue entitled: Proteomics, mass spectromety and peptidomics, Cancun 2013. Guest Editors: Cesar Lopez-Camarillo, Victoria Pando-Robles and Bronwyn Jane Barlda. (C) 2014 Elsevier B.V. All rights reserved.