Novel Method for Continuous Cell Separation by Density Gradient Centrifugation: Evaluation of a Miniature Separation Column
Novel Method for Continuous Cell Separation by Density Gradient Centrifugation: Evaluation of a Miniature Separation Column
复制标题
密度梯度离心连续细胞分离的新方法：微型分离柱的评估
DOI：
10.1081/pb-120021434
复制
发表时间：
2003
影响因子：
2.9
通讯作者：
Y. Ito
中科院分区：
文献类型：
--
作者：
H. Shiono;Y. Ito
摘要小型台式离心机可根据密度差异进行连续细胞分离。该装置装有一个小的分离盘，该分离盘配备有一个由隔膜隔开的圆形通道（8 mL容量）。一组具有不同密度的等渗Percoll介质在一个终端连续引入并从另一个终端收集。在离心力场下，引入通道近端部分的细胞悬浮液导致细胞根据其密度连续分离。该装置的性能通过在大群（1010）RBC中分离含有有核细胞（>108）的人血沉棕黄层来证明。结果表明，该方法能够分离大量的有核细胞，损伤最小，操作数小时，其中嗜中性粒细胞与淋巴细胞很好地分离。该方法可应用于其他类型的样品，包括脐带血、来自小动物的血液、培养的细胞、胰岛β细胞、疟疾寄生虫、精子细胞等。
Abstract A compact bench-top model of the centrifuge enables continuous cell separation based on density differences. The apparatus holds a small separation disk equipped with a circular channel (8 mL capacity) separated by a septum. A set of isotonic Percoll media with different densities is continuously introduced at one terminal and collected from the other. Under a centrifugal force field, cell suspension introduced into the proximal portion of the channel results in continuous separation of cells according to their densities. The performance of the apparatus was demonstrated with the separation of human buffy coat containing nucleated cells (>108) among a large population (1010) of RBC. The results indicated that the method is capable of separating a large number of nucleated cells, with minimum damage, for a few hours of operation wherein neutrophils are well resolved from lymphocytes. The method may be applied to other types of samples including cord blood, blood from small animals, cultured cells, pancreatic β cell islets, malaria parasites, sperm cells, etc.