A universal method for the speciation analysis of arsenic in various seafood based on microwave-assisted extraction and ion chromatography-inductively coupled plasma mass spectrometry
A universal method for the speciation analysis of arsenic in various seafood based on microwave-assisted extraction and ion chromatography-inductively coupled plasma mass spectrometry
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基于微波辅助萃取和离子色谱-电感耦合等离子体质谱分析各种海鲜中砷形态的通用方法
DOI：
10.1016/j.microc.2020.105592
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发表时间：
2020-12-01
影响因子：
4.8
通讯作者：
Fu, FengFu
中科院分区：
文献类型：
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作者：
Lin, Yaohui;Sun, Ying;Fu, FengFu
海产品中砷的形态分析对客观、科学地评价海产品中砷的健康风险具有重要意义。一般来说，不同种类的海鲜含有多种不同种类的砷，其基质也有很大的不同。因此，对不同种类海产品中砷的检测需要不同的提取和分析方法。本研究建立了一种通用的微波辅助提取方法，用于提取各种海产品中砷(V)、As（III）、一甲基胂酸（MMA）、二甲基胂酸（DMA）、砷甜菜碱（AsB）、砷糖等砷，提取效率为95%，且不改变其原始种类。建立了通用阳离子交换IC-ICP-MS（离子色谱-电感耦合等离子体质谱）检测海产品（海藻、鱼类、贝类和虾类）中砷的方法，方法检出限为8.0 ng As/g海产品干-12.0 ng As/g海产品干。特别是，微波辅助提取得到的海产品提取物可直接用于IC-ICP-MS检测，无需任何预处理。采用萃取法和IC-ICP-MS法，成功检测了海藻、鱼类、贝类和虾类样品中的As(V)、As（III）、MMA、DMA、AsB、砷糖等砷，回收率为92% ~ 104%，相对标准偏差(RSD, n = 5) < 5%。所建立的微波辅助提取法和IC-ICP-MS检测法操作简单、适用范围广、效率高、准确度好、对复杂基质的抵抗能力强，为客观、科学地评估各种海产品中砷的健康风险提供了可靠的方法，保证了海产品的消费安全。
The speciation analysis of arsenic in seafood is of great significance for objectively and scientifically assessing arsenic health risks in seafood. In general, different types of seafood contain a variety of different arsenic species and have quite different matrix. Thus, the detection of arsenic species in different types of seafood required different extracting and analytical methods. In this study, a universal microwave-assisted extraction was developed to extract each arsenic species including As(V), As(III), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenobetaine (AsB), arsenosugars and so on in various seafood with a extraction efficiency 95% and without altering their original species, and a universal cation exchange IC-ICP-MS (ion chromatography-inductively coupled plasma mass spectrometry) method was developed for the detection of arsenic species in various seafood (seaweed, fish, shellfish and shrimp) with a method detection limit of 8.0 ng As/g dried seafood-12.0 ng As/g dried seafood. Especially, the extract of seafood obtained with our microwave assisted extraction can be directly used for the following IC-ICP-MS detection without any additional pretreatment. By using the extraction method and IC-ICP-MS method, we have successfully detected each arsenic species including As(V), As(III), MMA, DMA, AsB, arsenosugars and so on in seaweed, fish, shellfish and shrimp samples with a recovery of 92%-104% and a relative standard deviation (RSD, n = 5) < 5%. The developed microwave assisted extraction method and IC-ICP-MS detection method is simple, has broad applicability, high efficiency, excellent accuracy and strong resistibility to the complicated matrix, which promising a reliable approach for objectively and scientifically assessing arsenic health risks in various seafood to ensure the consumption safety of seafood.