Ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometric procedure for qualitative and quantitative analyses of nortriterpenoids and lignans in the genus Schisandra.
Ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometric procedure for qualitative and quantitative analyses of nortriterpenoids and lignans in the genus Schisandra.
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DOI：
10.1016/j.jpba.2011.07.045
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发表时间：
2011-12
影响因子：
3.4
通讯作者：
Yan Zhou;Sheng-Xiong Huang;J. Pu;Jinrong Li;L. Ding;Dao-feng Chen;Han-Dong Sun;Hong-Xi Xu
中科院分区：
文献类型：
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作者：
Yan Zhou;Sheng-Xiong Huang;J. Pu;Jinrong Li;L. Ding;Dao-feng Chen;Han-Dong Sun;Hong-Xi Xu
建立了超高效液相色谱-四极杆飞行时间质谱联用技术，首次同时分析五味子中的降三萜和木脂素类化合物。该方法包括三个单独的质谱实验，包括全扫描MS，MS/MS实验和源内碰撞诱导解离（CID）MS/MS，这使得能够识别诊断裂解途径的nortriperenoids和木脂素。因此，在12.5min内，共明确鉴定出6种去甲三萜和10种木脂素，并初步从不同的五味子样品中鉴定出1种去甲三萜和20种木脂素。另外，在48个样品中还检测到6种降三萜和10种木脂素。chinensis和S.使用全扫描MS实验的提取物离子色谱图（XIC）对sphenanthera进行鉴定。采用主成分分析（PCA）和正交偏最小二乘判别分析（OPLS-DA）对超高效液相色谱-质谱联用（UPLC-MS）数据进行处理，比较两种五味子属植物的差异。
An ultra performance liquid chromatography (UPLC) coupled with quadrupole time-of-flight mass spectrometry (QTOF-MS) procedure is designed for the first simultaneous analysis of nortriterpenoids and lignans in Schisandra samples. The method consists of three individual mass spectrometric experiments, including the full scan MS, MS/MS experiment and in-source collision induced dissociation (CID) MS/MS, which enable the identification of diagnostic fragmentation pathways of nortriterpenoids and lignans. As such, a total of 6 nortriterpenoids and 10 lignans were unequivocally identified, and one nortriterpenoid and 20 lignans were tentatively identified from different Schisandra samples within 12.5min. In addition, 6 nortriterpenoids and 10 lignans were quantified in 48 samples of S. chinensis and S. sphenanthera using an extract ion chromatogram (XIC) of the full scan MS experiment. Dataset obtained from UPLC–MS was processed with principal component analysis (PCA) and orthogonal partial least squared discriminant analysis (OPLS-DA) to compare the difference between the two Schisandra species.