DFL1, an auxin-responsive GH3 gene homologue, negatively regulates shoot cell elongation and lateral root formation, and positively regulates the light response of hypocotyl length
DFL1, an auxin-responsive GH3 gene homologue, negatively regulates shoot cell elongation and lateral root formation, and positively regulates the light response of hypocotyl length
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DOI：
10.1046/j.1365-313x.2001.00957.x
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发表时间：
2001-01-01
期刊：
影响因子：
7.2
通讯作者：
Matsui, M
中科院分区：
文献类型：
--
作者：
Nakazawa, M;Yabe, N;Matsui, M
从1200个拟南芥激活标记株系中筛选到一个新的显性突变体dfl 1-D。dfl 1-D在蓝光、红光和远红光下下胚轴较短，但在黑暗下不短，抑制芽中细胞伸长导致成年植株的过度矮化表型。dfl 1-D对侧根生长有抑制作用，但对主根长度无影响。通过质粒拯救法克隆与T-DNA右边界相邻的基因组DNA。拯救的基因组DNA含有编码GH 3同源物的基因。转录本在dfl 1-D中高度积累。dfl 1-D表型通过该基因在野生型植物中的过表达得到证实。dfl 1-D对外源生长素处理表现抗性。此外，过表达的反义DFL 1导致更大的芽和侧根的数量增加。这些结果表明，DFL 1基因产物参与了生长素信号转导，并在光照条件下抑制了芽和下胚轴细胞的伸长以及侧根细胞的分化。
A novel dominant mutant designated 'dwarf in light 1' (dfl1-D) was isolated from screening around 1200 Arabidopsis activation-tagged lines. dfl1-D has a shorter hypocotyl under blue, red and far-red light, but not in darkness, inhibition of cell elongation in shoots caused an exaggerated dwarf phenotype in the adult plant. The lateral root growth of dfl1-D was inhibited without any reduction of primary root length. The genomic DNA adjacent to the right border of T-DNA was cloned by plasmid rescue. The rescued genomic DNA contained a gene encoding a GH3 homologue. The transcript was highly accumulated in the dfl1-D. The dfl1-D phenotype was confirmed by over-expression of the gene in the wild-type plant. The dfl1-D showed resistance to exogenous auxin treatment. Moreover, over-expression of antisense DFL1 resulted in larger shoots and an increase in the number of lateral roots. These results indicate that the gene product of DFL1 is involved in auxin signal transduction, and inhibits shoot and hypocotyl cell elongation and lateral root cell differentiation in light.