Cloning of the Pachysolen tannophilus Xylulokinase Gene by Complementation in Escherichia coli
Cloning of the Pachysolen tannophilus Xylulokinase Gene by Complementation in Escherichia coli
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DOI：
10.1128/aem.53.12.2975-2977.1987
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发表时间：
1987-12
影响因子：
4.4
通讯作者：
P. Stevis;James J. Huang;N. Ho
中科院分区：
文献类型：
--
作者：
P. Stevis;James J. Huang;N. Ho
通过与大肠杆菌木糖激酶（xyylb）突变体的互补，从酵母菌Pachysolen tannophilus中分离到了木糖激酶的编码基因。通过亚克隆，该基因被定位在一个3.2千碱基EcoRI-PstI片段的一端。克隆基因的表达对葡萄糖抑制不敏感。此外，克隆的基因与大肠杆菌和酿酒酵母的木糖激酶基因没有交叉杂交。
The gene coding for xylulokinase has been isolated from the yeast Pachysolen tannophilus by complementation of Escherichia coli xylulokinase (xylB) mutants. Through subcloning, the gene has been localized at one end of a 3.2-kilobase EcoRI-PstI fragment. Expression of the cloned gene was insensitive to glucose inhibition. Furthermore, the cloned gene did not cross-hybridize with E. coli and Saccharomyces cerevisiae xylulokinase genes.