Postinjury niches induce temporal shifts in progenitor fates to direct lesion repair after spinal cord injury.
Postinjury niches induce temporal shifts in progenitor fates to direct lesion repair after spinal cord injury.
复制标题
DOI：
10.1523/jneurosci.4538-08.2009
复制
发表时间：
2009-05-20
期刊：
影响因子：
--
通讯作者：
Horner PJ
中科院分区：
文献类型：
--
作者：
Sellers DL;Maris DO;Horner PJ
Progenitors that express NG2-proteoglycan are the predominant self-renewing cell within the CNS. NG2-progenitors replenish oligodendrocyte populations within the intact stem-cell niche, and cycling NG2-cells are among the first cells to react to CNS insults. We investigated the role of NG2-progenitors after spinal cord injury (SCI) and how bone morphogen protein (BMP) signals remodel the progressive post-injury niche. Progeny labeled by an NG2-specific reporter virus undergo a coordinated shift in differentiation profile. NG2-progeny born 24-hours post-injury (PI) produce scar-forming astrocytes and transient populations of novel phagocytic astrocytes shown to contain denatured myelin within cathepsin-D labeled endosomes, but NG2-progenitors born 7-days PI differentiate into oligodendrocytes and express myelin on processes that wrap axons. Analysis of spinal cord mRNA shows a temporal-shift in the niche-transcriptome of ligands that affect post-injury remodeling and direct progenitor differentiation. We conclude that NG2-progeny are diverse lineages that obey progressive-cues after trauma to replenish the injured niche.
