Comparison of enzyme immunoassay and immunodiffusion for the detection of canine blastomycosis.
Comparison of enzyme immunoassay and immunodiffusion for the detection of canine blastomycosis.
复制标题
酶免疫分析法与免疫扩散法检测犬芽生菌病的比较。
DOI：
10.1111/myc.1990.33.7-8.375
复制
发表时间：
1990
期刊：
影响因子：
4.9
通讯作者：
Scalarone,GM
中科院分区：
文献类型：
--
作者：
Seawell,BW;Scalarone,GM
两种芽生菌皮炎非商业免疫扩散抗原，Meridian Diagnostics和Nolan-Scott Laboratories，以及两种B.将我们实验室制备的皮炎酵母相裂解抗原T-58和K-Le用于酶联免疫吸附试验（ELISA），以检测61份芽生菌病犬血清标本中的抗体。将ELISA中使用的所有抗原稀释至50 ng蛋白ml-1。还使用Nolan-Scott ID抗原对血清进行免疫扩散（ID）试验。基于ELISA指数值，在ELISA中，两种酵母相裂解物抗原的反应性高于两种市售试剂。此外，使用T-58、K-Le和Meridian抗原的ELISA的灵敏度显著高于ID试验。然而，当用于ID程序时，Nolan-Scott抗原能够检测到比ELISA更多的阳性标本。因此，关于使用B的ELISA的持续开发和优化获得了令人鼓舞的结果。用于诊断芽生菌病的皮炎细胞裂解物抗原。
TwoBlastomyces dermatitidiscommercial immunodiffusion antigens, Meridian Diagnostics and Nolan‐Scott Laboratories, and twoB. dermatitidisyeast phase lysate antigens, T‐58 and K‐Le, prepared in our laboratory were utilized in an enzyme‐linked immunosorbent assay (ELISA) for the detection of antibodies in 61 serum specimens from dogs with blastomycosis. All antigens used in the ELISA were diluted to 50 ng of protein ml−1. Immunodiffusion (ID) tests were also performed on the sera using a Nolan‐Scott ID antigen. Greater reactivity, based on the ELISA index value, was evidenced with the two yeast phase lysate antigens over the two commercial reagents in the ELISA. In addition the sensitivity of the ELISA with the T‐58, K‐Le and Meridian antigens was considerably greater than that of the ID test. The Nolan‐Scott antigen, however, was able to detect more positive specimens when used in the ID procedure than in the ELISA. Therefore encouraging results were obtained with respect to the continued development and optimization of the ELISA usingB. dermatitidiscell lysate antigens for the diagnosis of blastomycosis.