Roles in dimerization and blue light photoresponse of the PAS and LOV domains of Neurospora crassa white collar proteins
Roles in dimerization and blue light photoresponse of the PAS and LOV domains of Neurospora crassa white collar proteins
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DOI：
10.1046/j.1365-2958.1998.00955.x
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发表时间：
1998-08-01
影响因子：
3.6
通讯作者：
Macino, G
中科院分区：
文献类型：
--
作者：
Ballario, P;Talora, C;Macino, G
之前已分离出编码白领1和白领2（wc-1和we-2）的基因，其产物被表征为锌指转录因子，参与蓝光诱导基因的控制。在这里，我们证明两种蛋白中存在的PAS二聚化结构域使WC-1和WC-2蛋白能够在体外二聚化，白领（WC）之间形成同二聚体和异二聚体 蛋白质，WC-1 的计算机分析揭示了第二个结构域，称为 LOV，也在 NPH1 中发现，NPH1 是植物中假定的蓝光感受器，在氧化还原敏感蛋白和光敏色素中保守，WC-1 LOV 结构域不与典型的 PAS 结构域二聚化，但它能够自我二聚化，三个盲 WC-1 菌株的分离，每个菌株仅具有单个氨基酸取代 这些LOV突变体中的WC-1蛋白仍然能够自我二聚化，表明该结构域在蓝光信号转导中发挥着额外的作用。
The genes coding for white collar-1 and white collar-2 (wc-1 and we-2) have been isolated previously, and their products characterized as Zn-finger transcription factors involved in the control of blue light-induced genes, Here, we show that the PAS dimerization domains present in both proteins enable the WC-1 and WC-2 proteins to dimerize in vitro, Homodimers and heterodimers are formed between the white collar (WC) proteins, A computer analysis of WC-1 reveals a second domain, called LOV, also identified in NPH1, a putative blue light photoreceptor in plants and conserved in redox-sensitive proteins and in the phytochromes, The WC-1 LOV domain does not dimerize with canonical PAS domains, but it is able to self-dimerize, The isolation of three blind wc-1 strains, each with a single amino acid substitution only in the LOV domain, reveals that this region is essential for blue light responses in Neurospora, The demonstration that the WC-1 proteins in these LOV mutants are still able to self-dimerize suggests that this domain plays an additional role, essential in blue light signal transduction.