Quantification of hepatic thrombopoietin mRNA transcripts in patients with chronic liver diseases shows maintained gene expression in different etiologies of liver cirrhosis.
Quantification of hepatic thrombopoietin mRNA transcripts in patients with chronic liver diseases shows maintained gene expression in different etiologies of liver cirrhosis.
复制标题
对慢性肝病患者肝血小板生成素 mRNA 转录本的定量显示，在不同病因的肝硬化中基因表达保持不变。
DOI：
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
P. Schöffski
中科院分区：
文献类型：
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作者：
F. Tacke;C. Trautwein;Shourong Zhao;M. Andreeff;M. Manns;A. Ganser;P. Schöffski
背景/目的
血小板生成受血小板生成素（TPO）调节，血小板生成素主要在肝脏中合成。肝脏疾病患者的TPO可能是由于肝脏TPO产生受损所致。正如我们先前报道的，与健康对照组相比，肝病患者的TPO血清水平没有降低，并且不依赖于肝硬化的阶段或血小板计数，但在慢性病毒性肝炎患者中高度升高。
方法
为了研究其可能的机制，我们采用TaqMan实时荧光定量RT-PCR技术检测了31例肝硬化患者肝组织中TPO mRNA的水平，并采用ELISA法检测了相应的血清TPO浓度。
结果
与胆道（n = 10）、酒精（n = 6）或其他（n = 3）疾病病因的患者相比，病毒性肝炎患者（n = 12）的中位TPO血清水平升高，而肝脏TPO mRNA水平无差异。慢性肝病患者肝组织TPO mRNA水平与正常肝组织比较无显著性差异。TPO mRNA与血清TPO水平无相关性。
结论
我们的结论是，肝TPO基因的表达似乎是维持在一个组成性的转录水平上的肝病患者，并不改变依赖于疾病的病因。
BACKGROUND/AIMS
Platelet production is regulated by thrombopoietin (TPO), which is primarily synthesized in the liver. The TPO in patients with liver diseases could possibly be owing to impaired hepatic TPO production. As we reported previously, TPO serum levels are not decreased in patients with liver diseases compared with healthy controls and do not depend on the stage of cirrhosis or platelet count, but are highly elevated in patients with chronic virus hepatitis.
METHODS
To study possible mechanisms, we measured hepatic TPO mRNA levels in liver tissue samples from 31 liver cirrhosis patients by quantitative TaqMan real-time RT-PCR and corresponding serum TPO concentrations by ELISA.
RESULTS
Median TPO serum levels were elevated in patients with viral hepatitis (n = 12) compared with patients with a biliary (n = 10), alcoholic (n = 6) or other (n = 3) disease etiology, while hepatic TPO mRNA levels did not differ. The TPO mRNA levels in patients with chronic liver diseases were not different from normal liver tissue sample. The TPO mRNA and TPO serum level did not correlate.
CONCLUSIONS
We conclude that hepatic TPO gene expression appears to be maintained on a constitutive transcriptional level in patients with liver diseases and does not change dependent on disease etiology.