Paenibacillus polymyxa produces fusaricidin-type antifungal antibiotics active against Leptosphaeria maculans, the causative agent of blackleg disease of canola
Paenibacillus polymyxa produces fusaricidin-type antifungal antibiotics active against Leptosphaeria maculans, the causative agent of blackleg disease of canola
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DOI：
10.1139/w02-002
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发表时间：
2002-02-01
影响因子：
2.8
通讯作者：
Jensen, SE
中科院分区：
文献类型：
--
作者：
Beatty, PH;Jensen, SE
一种能够抑制斑点细球腔菌（Leptosphaeria maculans（Desmaz.）塞斯& De Not.，甘蓝型油菜黑胫病的病原菌是油菜黑胫病的病原菌。和芜菁（Brassica rapa L.），被认为是一种潜在的生物控制剂。基于其（i）生物化学和生长特征和（ii）16 S rRNA序列相似性，该环境分离物被确定为多粘类芽孢杆菌，并被赋予菌株名称PKB 1。抗真菌肽是由多粘类原杆菌PKB 1在孢子形成开始时产生的，在马铃薯葡萄糖肉汤中的产量最佳。用甲醇从P. polymyxa PKB 1细胞和（或）孢子中提取抗真菌肽，并使用尺寸排阻和反相色谱进行纯化。抗真菌肽的表征是使用氨基酸组成分析，Edman降解测序从部分水解的材料，和各种质谱方法。发现纯化的抗真菌物质是分子量为883、897、948和961 Da的相关肽的混合物，其中897-Da组分的最可能结构确定为具有与游离氨基结合的不寻常的15-胍基-3-羟基十五烷酸部分的环状缩酚酸肽。因此，这些化合物是环状缩酚肽的杀镰孢菌素组的成员。
A bacterial isolate capable of inhibiting the growth of Leptosphaeria maculans (Desmaz.) Ces. & De Not., the causative agent of blackleg disease of canola (Brassica napus L. and Brassica rapa L.), was identified as a potential biological control agent. This environmental isolate was determined to be Paenibacillus polymyxa based on its (i) biochemical and growth characteristics and (ii) 16S rRNA sequence similarity, and was given the strain designation PKB1. Antifungal peptides were produced by P. polymyxa PKB1 around the onset of sporulation, with optimal production on potato dextrose broth. The antifungal peptides were extracted from P. polymyxa PKB1 cells and (or) spores using methanol and were purified using size exclusion and reverse-phase chromatography. Characterization of the antifungal peptides was done using amino acid compositional analysis, Edman degradation sequencing from partially hydrolyzed material, and a variety of mass spectrometric methods. The purified antifungal material was found to be a mixture of related peptides of molecular masses 883, 897, 948, and 961 Da, with the most likely structure of the 897-Da component determined to be a cyclic depsipeptide with an unusual 15-guanidino-3-hydroxypentadecanoic acid moiety bound to a free amino group. These compounds are therefore members of the fusaricidin group of cyclic depsipeptides.