Hydrogel based on interpenetrating polymer networks of dextran and gelatin for vascular tissue engineering
Hydrogel based on interpenetrating polymer networks of dextran and gelatin for vascular tissue engineering
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DOI：
10.1016/j.biomaterials.2008.09.041
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发表时间：
2009-01-01
期刊：
影响因子：
14
通讯作者：
Chan-Park, Mary B.
中科院分区：
文献类型：
--
作者：
Liu, Yunxiao;Chan-Park, Mary B.
由于水凝胶网络具有高含水量和模仿天然细胞外基质的能力，因此非常适合作为细胞封装的三维（3-D）组织工程支架。然而，它们的应用受到纳米级网格尺寸的限制，限制了封装细胞的扩散和增殖，而且它们的机械性能较差。本研究旨在通过应用新型细胞封装水凝胶家族来解决这两个局限性，该家族基于明胶和葡聚糖的互穿聚合物网络（IPN），并用甲基丙烯酸酯（MA）和醛（AD）双功能化（Dex-MA-AD）。通过H-1-NMR验证了合成的Dex-MA-AD的化学结构，发现MA和AD的取代度分别为14和13.9+/-1.3。所有这些水凝胶中的水含量约为80%。向纯 Dex-MA-AD 中添加 40 mg/ml 至 60 mg/ml 明胶将压缩模量从 15.4 +/- 3.0 kPa 增加到约 51.9 +/- 0.1 kPa（约 3.4 倍）。此外，我们的 IPN 水凝胶比常用于平滑肌细胞 (SMC) 封装的聚乙二醇水凝胶（约 10(2)-10(3) Pa）具有更高的动态储能模量（即约 10(4) Pa）。我们的基于葡聚糖的 IPN 水凝胶不仅支持内皮细胞 (EC) 在表面上的粘附和扩散，而且还允许封装的 SMC 在水凝胶的内部增殖和扩散。这些 IPN 水凝胶作为血管组织工程的 3D 支架似乎很有前景。 (c) 2008 Elsevier Ltd. 保留所有权利。
Hydrogel networks are highly desirable as three-dimensional (3-D) tissue engineering scaffolds for cell encapsulation due to the high water content and ability to mimick the native extracellular matrix. However, their application is limited by their nanometer-scale mesh size, which restricts the Spreading and proliferation of encapsulated cells, and their poor mechanical properties. This study seeks to address both limitations through application of a novel cell-encapsulating hydrogel family based on the interpenetrating polymer network (IPN) of gelatin and dextran bifunctionalized with methacrylate (MA) and aldehyde (AD) (Dex-MA-AD). The chemical Structure of the synthesized Dex-MA-AD was verified by H-1-NMR and the degrees of substitution of MA and AD were found to be 14 and 13.9 +/- 1.3 respectively. The water contents in all these hydrogels were approximately 80%. Addition of 40 mg/ml to 60 mg/ml gelatin to neat Dex-MA-AD increased the compressive modulus from 15.4 +/- 3.0 kPa to around 51.9 +/- 0.1 kPa (about 3.4-fold). Further, our IPN hydrogels have higher dynamic storage moduli (i.e. on the order of 10(4) Pa) than polyethylene glycol-based hydrogels (around 10(2)-10(3) pa) commonly used for smooth muscle cells (SMCs) encapsulation. Our dextran-based IPN hydrogels not only supported endothelial cells (ECs) adhesion and spreading on the surface, but also allowed encapsulated SMCs to proliferate and spread in the bulk interior of the hydrogel. These IPN hydrogels appear promising as 3-D scaffolds for vascular tissue engineering. (c) 2008 Elsevier Ltd. All rights reserved.