Activation of protein kinase C inhibits adenovirus VA gene transcription in vitro.
Activation of protein kinase C inhibits adenovirus VA gene transcription in vitro.
复制标题
蛋白激酶 C 的激活可抑制体外腺病毒 VA 基因的转录。
DOI：
10.1099/0022-1317-73-12-3133
复制
发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Carter,TH
中科院分区：
文献类型：
--
作者：
James,CB;Carter,TH
We report here that activation of protein kinase C (PKC) results in the inhibition of adenovirus virus-associated (VA) gene transcriptionin vitro. The involvement of PKC in this inhibition is supported by the fact that the addition of PKC inhibitors to transcription reactions in which the PKC cofactor phosphatidyl serine (PS) was present resulted in increased levels of transcription compared to those in reactions in which PKC activity was stimulated in the absence of PKC inhibitors. Furthermore, based on thesein vitrostudies we propose that the inhibition of VA gene transcription is possibly due to the inability of the VA gene to form an active transcription complex following the activation of PKC. This conclusion was drawn fromin vitrodata which demonstrated that PS stimulation of endogenous PKC present in cell extracts resulted in failure to isolate a fully initiated, stable transcription complex.
