Two dopaminergic neurons signal to the dorsal fan-shaped body to promote wakefulness in Drosophila.
Two dopaminergic neurons signal to the dorsal fan-shaped body to promote wakefulness in Drosophila.
复制标题
两种多巴胺能神经元向背扇形身体发出信号，以促进果蝇的清醒。
DOI：
10.1016/j.cub.2012.09.008
复制
发表时间：
2012-11-20
期刊：
影响因子：
9.2
通讯作者：
Wu, Mark N.
中科院分区：
文献类型：
--
作者：
Liu, Qili;Liu, Sha;Kodama, Lay;Driscoll, Maria R.;Wu, Mark N.
人们对睡眠背后的神经回路知之甚少。尽管多巴胺 (DA) 被认为在睡眠/觉醒调节中发挥着关键作用，但该过程所需的单个 DA 神经元及其下游靶标的身份尚不清楚。在这里，我们在每个 PPL1 簇中识别出一个促进果蝇觉醒的 DA 神经元。成像数据表明，这些神经元的活动在清醒时增加，这与促进觉醒的作用一致。引人注目的是，这些神经元投射到背侧扇形体，此前已被证明可以促进睡眠。 DA 神经元激活引起的睡眠减少可以通过 DopR 的缺失来阻断，扇形体中 DopR 表达的恢复可以挽救 DopR 突变背景中 DA 的促醒作用。这些实验在单细胞水平上定义了一种新颖的唤醒回路。由于背侧扇形体促进睡眠，这些数据提供了唤醒和睡眠回路之间的关键联系。此外，这些发现表明，通过单胺能信号传导抑制睡眠中枢是一种进化上保守的促进觉醒的机制。
The neuronal circuitry underlying sleep is poorly understood. Although dopamine (DA) is thought to play a key role in sleep/wake regulation, the identities of the individual DA neurons and their downstream targets required for this process are unknown. Here, we identify a DA neuron in each PPL1 cluster that promotes wakefulness in Drosophila. Imaging data suggest that the activity of these neurons is increased during wakefulness, consistent with a role in promoting arousal. Strikingly, these neurons project to the dorsal fan-shaped body, which has previously been shown to promote sleep. The reduced sleep caused by activation of DA neurons can be blocked by loss of DopR, and restoration of DopR expression in the fan-shaped body can rescue the wake-promoting effects of DA in a DopR mutant background. These experiments define a novel arousal circuit at the single cell level. Since the dorsal fan-shaped body promotes sleep, these data provide a key link between wake and sleep circuits. Furthermore, these findings suggest that inhibition of sleep centers via monoaminergic signaling is an evolutionarily conserved mechanism to promote arousal.
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