Site-directed mutageneses of rat liver cytochrome P-450d: axial ligand and heme incorporation.
Site-directed mutageneses of rat liver cytochrome P-450d: axial ligand and heme incorporation.
复制标题
大鼠肝细胞色素 P-450d 的定点诱变：轴向配体和血红素掺入。
作者：
Toru Shimizu;K. Hirano;Masae Takahashi;M. Hatano;Y. Fujii‐Kuriyama
用谷胱甘肽定向诱变法，对大鼠肝细胞色素P-450 d的羧基端进行了13个氨基酸的替换：（A）Phe-449-Tyr;（B）Gly-450-Ser;（C）Leu-451-Ser;（D）Gly-452-Glu;（E）Lys-453-Glu;（F）Arg-454-Leu;（G）Arg-455-Gly;（H）Cys-456-Tyr;（I）Cys-456-His;（J）Ile-457-Ser;（K）Gly-458-Glu;（L）Glu-459-Ala;（M）Ile-460-Ser。突变体A的CO络合物在445 nm处产生Soret峰。与野生型复合物相比，突变体A、C、D和J的CO结合形式的强度非常小。突变体H、I和K的CO还原形式在450 nm附近根本不产生Soret峰。突变体F的448-nm峰不稳定，并迅速消失，同时在420 nm处出现一个峰。（250字处删节）
By oligonucleotide-directed mutageneses, 13 substitutions of amino acids at the carboxy-terminal region of rat liver cytochrome P-450d were done as follows: (A) Phe-449----Tyr; (B) Gly-450----Ser; (C) Leu-451----Ser; (D) Gly-452----Glu; (E) Lys-453----Glu; (F) Arg-454----Leu; (G) Arg-455----Gly; (H) Cys-456----Tyr; (I) Cys-456----His; (J) Ile-457----Ser; (K) Gly-458----Glu; (L) Glu-459----Ala; (M) Ile-460----Ser. The CO-bound reduced forms of the wild type and mutants B-G, J, L, and M gave Soret peaks at 448 nm. The CO complex of mutant A gave a Soret peak at 445 nm. The intensities of the CO-bound forms of mutants A, C, D, and J were very small compared with that of the wild-type complex. The CO-reduced forms of mutants H, I, and K did not give a Soret peak around 450 nm at all. The 448-nm peak of mutant F was unstable and quickly disappeared with the concomitant appearance of a peak at 420 nm.(ABSTRACT TRUNCATED AT 250 WORDS)
影响因子：
2.9
作者：
POULOS, TL;FINZEL, BC;HOWARD, AJ
通讯作者：
HOWARD, AJ
肝微粒体细胞色素 P-450 同工酶 2 的结构特征。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Black,SD;Tarr,GE;Coon,MJ
通讯作者：
Coon,MJ