Urotensin-II expression in the mouse spinal cord
Urotensin-II expression in the mouse spinal cord
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DOI：
10.1016/j.jchemneu.2005.10.004
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发表时间：
2006-02-01
影响因子：
2.8
通讯作者：
Calas, A
中科院分区：
文献类型：
--
作者：
Egginger, JG;Camus, A;Calas, A
尾张力素- ii （UII）是一种12个氨基酸的肽，在硬骨鱼的神经分泌细胞中被发现，该细胞位于脊髓尾端，并投射到称为尿生理的神经血管腺。UII及其pre - pro-UII mRNA的分布并不局限于鱼类，例如在大鼠脊髓中也有发现。鉴于获得转基因小鼠的潜在兴趣，我们因此分别通过原位杂交和免疫组织化学在小鼠脊髓中表征了小鼠pro-UII mRNA和UII免疫反应性的分布。在不同节段的腹角处有一群uii样免疫反应细胞体。这些细胞表现出运动神经元的所有特征，双重免疫组织化学标记显示UII和囊泡乙酰胆碱转运蛋白共存，电镜免疫细胞化学证实了这一点。对支配球海绵体阴茎肌的运动神经元进行逆行标记，发现部分运动神经元含有UII。原位杂交组织化学显示，部分腹角神经元核周有pro-UII mRNA。含有pro-UII mrna的细胞体具有相同的运动神经元特征，证实了免疫组化研究的结果，表明小鼠UII基因在脊髓运动神经元亚群中表达。我们的研究结果支持了以鱼类内分泌为特征的ii肽也在哺乳动物运动神经元中表达的假设。(c) 2005 Elsevier B.V.版权所有
Urotensin-II (UII), a 12 amino acid peptide, was discovered in the teleost fish neurosecretory cells located in the caudal portion of the spinal cord and which project to a neurohemal gland called the urophysis. The distribution of UII and of its prepro-UII mRNA is not limited to fish and was found for example in the rat spinal cord. In view of the potential interest of obtaining transgenic mice, we have therefore characterized the distribution of mouse pro-UII mRNA and UII immunoreactivity, by in situ hybridization and immunohistochemistry, respectively, in the mouse spinal cord. A population of UII-like immunoreactive cell bodies was located in the ventral horn of the different segments. These cells displayed all the features of motoneurons, as confirmed by a double immunohistochemical labelling showing the co-occurrence of UII and vesicular acetylcholine transporter, and by electron microscope immunocytochemistry. Retrograde labelling of motoneurons innervating the bulbocavernosus penile muscle showed that some of them contained UII. In situ hybridization histochemistry revealed that pro-UII mRNA was located in some ventral horn neuronal perikarya. The pro-UII mRNA-containing cell bodies possessed the same motoneuron characteristics, confirming the results of the immunohistochemical studies and showing that the gene of mouse UII is expressed in a subpopulation of motoneurons in the spinal cord. Our results support the assumption that UII peptide characterized as endocrine in fish is also expressed within mammalian motoneurons. (c) 2005 Elsevier B.V. All rights reserved.