Elevated levels of leukotriene C4 in bronchoalveolar lavage fluid from atopic asthmatics after endobronchial allergen challenge.
Elevated levels of leukotriene C4 in bronchoalveolar lavage fluid from atopic asthmatics after endobronchial allergen challenge.
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DOI：
10.1164/ajrccm/142.1.112
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发表时间：
1990-07
期刊：
影响因子：
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通讯作者：
Sally E. Wenzel;Gary L. Larsen;Karen Johnston;Norbert F. Voelkel;Jay Y. Westcott
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作者：
Sally E. Wenzel;Gary L. Larsen;Karen Johnston;Norbert F. Voelkel;Jay Y. Westcott
磺胺多肽白三烯与哮喘的发病机制有关，因为它们能够诱导支气管痉挛、气道高反应性和粘液产生增加。本研究测定了非特应性非哮喘、非特应性哮喘、特应性非哮喘、特应性非哮喘和特应性哮喘四组患者支气管肺泡灌洗液（BALF）中白三烯（LT） C4、D4和E4的含量。经高效液相色谱法测定，经致敏原攻毒后，特应性哮喘患者半胱氨酸中主要的硫肽白三烯为LTC4。少量的LTD4和LTE4被检测到。特应性哮喘患者白三烯的基线水平为64 +/- 18 pg/ml，在11个样本中的9个样本中发现了可测量的水平。在7个基线样本中，特应性非哮喘患者只有一个样本的水平可测量，而6个非特应性受试者中有5个样本的水平无法检测到。异应性哮喘患者的过敏原刺激导致LTC4显著高于刺激前水平（64 +/- 18至616 +/- 193 pg/ml） （p < 0.01），刺激后三个对照组的LTC4显著高于刺激前水平（p = 0.0297）。在过敏原攻击后，特应性非哮喘组也检测到白三烯水平（88 +/- 32 pg/ml），而在6个非特应性样本中，有5个样本仍未检测到白三烯。为了比较，BALF还检测了组胺和前列腺素D2 （PGD2）、血栓素B2 （TxB2）。过敏原攻击后，过敏性哮喘患者的半胱氨酸中这三种介质的水平均升高。在过敏原攻击后，前列腺素PGD2和TxB2在BALF中的水平相关性最好（r = 0.88）。（摘要删节250字）
Sulfidopeptide leukotrienes have been implicated in the pathogenesis of asthma because of their ability to induce bronchospasm, airways hyperreactivity, and increased mucus production. In the present study, the leukotrienes (LT) C4, D4, and E4 were measured in bronchoalveolar lavage fluid (BALF) before and 5 min after endobronchial allergen challenge in four subject groups: nonatopic nonasthmatic, nonatopic asthmatic, atopic nonasthmatic, and atopic asthmatic. As determined by high performance liquid chromatography (HPLC), after allergen challenge, the predominant sulfidopeptide leukotriene found in BALF from atopic asthmatics was LTC4. Smaller amounts of LTD4 and LTE4 were detectable. The baseline level of leukotrienes in the atopic asthmatics was 64 +/- 18 pg/ml, with measurable levels being found in nine of 11 samples. Atopic nonasthmatics had measurable levels in only one of seven baseline samples, whereas five of six nonatopic subjects had undetectable levels. Allergen challenge in atopic asthmatics resulted in significant increases in LTC4 over prechallenge levels (64 +/- 18 to 616 +/- 193 pg/ml) (p less than 0.01) and over levels in the three control groups after challenge (p = 0.0297). The atopic nonasthmatic group also had detectable leukotriene levels after allergen challenge (88 +/- 32 pg/ml), whereas leukotrienes remained undetectable in five of the six nonatopic samples. For comparison, histamine and the prostanoids prostaglandin D2 (PGD2) and thromboxane B2 (TxB2) were also measured in BALF. The levels of all three of these mediators increased in BALF from atopic asthmatics after allergen challenge. After allergen challenge, the best correlation was found between the levels in BALF for the prostanoids PGD2 and TxB2 (r = 0.88).(ABSTRACT TRUNCATED AT 250 WORDS)