Specimen Preparation and Observations of Appressorial Cells Under Electron Microscopy
Specimen Preparation and Observations of Appressorial Cells Under Electron Microscopy
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附着细胞的标本制备及电镜观察
DOI：
10.1007/978-1-0716-1613-0_6
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发表时间：
2021
影响因子：
--
通讯作者：
Wilson, RA.
中科院分区：
文献类型：
--
作者：
Rocha, RO;Wilson, RA.
电子显微镜（EM）可以表征细胞的形态和超微结构。然而，关于冷冻样品固定的挑战仍然是其广泛采用的主要障碍之一。在本协议中，我们描述了两种可选的EM制备方法，用于在人工疏水表面上研究magnaporthe oryzaeapsoria。
Electron microscopy (EM) allows characterization of the morphology and ultrastructure of a cell. However, challenges concerning cryo sample fixation are still one of the main roadblocks to its widespread adoption. In this protocol, we describe two alternative EM preparation methods employed to studyMagnaporthe oryzaeappressoria on artificial hydrophobic surfaces.