Dynamic speckle illumination wide-field reflection phase microscopy.
Dynamic speckle illumination wide-field reflection phase microscopy.
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DOI：
10.1364/ol.39.006062
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发表时间：
2014-10-15
期刊：
影响因子：
3.6
通讯作者：
Yaqoob Z
中科院分区：
文献类型：
--
作者：
Choi Y;Hosseini P;Choi W;Dasari RR;So PT;Yaqoob Z
我们展示了一种基于时变散斑场照明的定量反射位相显微镜。由于散斑场的空间相干长度较短，该成像系统具有较高的横向分辨率(520 Nm)和高深度选择性(1.03µm)。离轴干涉检测可以实现适合于高速测量的广视场和单炮成像。此外，这种方法的测量相位灵敏度是可测量的最小轴向运动，比使用传输系统测得的相位灵敏度高40多倍。我们通过成功地区分红细胞顶面和底面的运动，证明了该方法的有效性。该方法可用于研究复杂真核细胞的膜动力学。
We demonstrate a quantitative reflection-phase microscope based on time-varying speckle-field illumination. Due to the short spatial coherence length of the speckle field, the proposed imaging system features superior lateral resolution, 520 nm, as well as high-depth selectivity, 1.03 µm. Off-axis interferometric detection enables wide-field and single-shot imaging appropriate for high-speed measurements. In addition, the measured phase sensitivity of this method, which is the smallest measurable axial motion, is more than 40 times higher than that available using a transmission system. We demonstrate the utility of our method by successfully distinguishing the motion of the top surface from that of the bottom in red blood cells. The proposed method will be useful for studying membrane dynamics in complex eukaryotic cells.