Fluorescence-amplifying detection of hydrogen peroxide with cationic conjugated polymers, and its application to glucose sensing
Fluorescence-amplifying detection of hydrogen peroxide with cationic conjugated polymers, and its application to glucose sensing
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阳离子共轭聚合物荧光放大检测过氧化氢及其在葡萄糖传感中的应用
DOI：
10.1002/adfm.200500602
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发表时间：
2006-01-05
影响因子：
19
通讯作者：
Zhu, DB
中科院分区：
文献类型：
--
作者：
He, F;Tang, YL;Zhu, DB
利用共轭聚合物的荧光猝灭效应，研制了一种高灵敏度的过氧化氢探针。采用阳离子共轭聚合物聚（9，9-双（6 '-N，N，N-三甲基铵-己基）芴亚苯基）（PFP-NMe ~（3+））和带有硼酸酯保护基的过氧氟-1（Fl-BB）对H_2O_2进行光学检测。在不添加H2 O2的情况下，阳离子PFP-NMe 3+和中性Fl-BB之间不存在静电相互作用，保持Fl-BB与PFP-NMe 3+良好分离，并且不发生PFP-NMC 3+的荧光猝灭。在H2 O2存在下，通过Fl-BB与H2 O2的特异性反应形成阴离子猝灭剂荧光素，导致PFP-NMe 3+和荧光素之间的强静电相互作用，因此发生PFPNMe 3+的有效荧光猝灭。荧光素的吸收与PFP-NMe 3+的发射重叠，这促进了从PFP-NMe 3+到荧光素的荧光共振能量转移（FRET）。H2 O2探针具有非常好的灵敏度，检测范围为15至600 nM。由于葡萄糖氧化酶（GOx）可以特异性地催化β-D-（+）-葡萄糖氧化生成H2 O2，因此也可以用H2 O2探针作为信号转导器来实现葡萄糖检测。
A highly sensitive hydrogen peroxide probe that takes advantage of the amplified fluorescence quenching of conjugated polymers has been developed. The cationic conjugated polymer, poly(9,9-bis(6'-N,N,N-trimethylammonium-hexyl) fluorene phenylene) (PFP-NMe3+) and peroxyfluor-1 with boronate protecting groups (Fl-BB) are used to detect H2O2 optically. Without the addition of H2O2, the absence of electrostatic interactions between the cationic PFP-NMe3+ and the neutral Fl-BB keeps the Fl-BB well separated from the PFP-NMe3+, and no fluorescence quenching of the PFP-NMC3+ occurs. In the presence of H2O2, the formation of the anionic quencher, fluorescein, by specific reaction of the Fl-BB with H2O2 results in strong electrostatic interactions between the PFP-NMe3+ and the fluorescein, and therefore efficient fluorescence quenching of the PFPNMe3+ occurs. The absorption of fluorescein overlaps the emission of PFP-NMe3+, which encourages fluorescence resonance energy transfer (FRET) from the PFP-NMe3+ to the fluorescein. The H2O2 probe has very good sensitivity, with a detection range of 15 to 600 nM. Since glucose oxidase (GOx) can specifically catalyze the oxidation of beta-D-(+)-glucose to generate H2O2, glucose detection is also realized with the H2O2 probe as the signal transducer.