Detection of influenza virus using a lateral flow immunoassay for amplified DNA by a microfluidic RT-PCR chip
Detection of influenza virus using a lateral flow immunoassay for amplified DNA by a microfluidic RT-PCR chip
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DOI：
10.1039/c2an16294f
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发表时间：
2012-01-01
期刊：
影响因子：
4.2
通讯作者：
Tamiya, Eiichi
中科院分区：
文献类型：
--
作者：
Nagatani, Naoki;Yamanaka, Keiichiro;Tamiya, Eiichi
用聚二甲基硅氧烷微流控芯片在15~20min内扩增出流感病毒RNA。用侧向流动免疫分析法在1min内用肉眼观察到扩增出的流感病毒RNA，在测试线上呈红色。
Influenza virus RNA was amplified by a continuous-flow poly-dimethylsiloxane microfluidic RT-PCR chip within 15-20 min. The amplified influenza virus RNA was observed with the naked eye, as the red color at the test line, using a lateral flow immunoassay within 1 min.