Ethanol-Extracted Brazilian Propolis Exerts Protective Effects on Tumorigenesis in Wistar Hannover Rats.
Ethanol-Extracted Brazilian Propolis Exerts Protective Effects on Tumorigenesis in Wistar Hannover Rats.
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DOI：
10.1371/journal.pone.0158654
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Wanibuchi H
中科院分区：
文献类型：
--
作者：
Kakehashi A;Ishii N;Fujioka M;Doi K;Gi M;Wanibuchi H
本研究用104周的时间来评估乙醇提取的巴西绿蜂胶(EEP)的致癌性。实验开始时6周龄的Wistar汉诺威大鼠，雄性和雌性各50只，暴露于饲料中0、0.5或2.5%剂量的EEP。0.5%和2.5%EEP处理的雄性和雌性大鼠的存活率分别显著高于各自的对照组。两年后对大鼠组织中肿瘤的总体组织病理学评估显示，给予EEP的动物的任何器官中的肿瘤或癌前病变都没有明显增加。0.5%EEP男性组和2.5%EEP女性组的垂体肿瘤、2.5%EEP治疗的男性和女性恶性淋巴瘤/白血病以及0.5%EEP男性组的全甲状腺肿瘤的发生率均显著降低。2.5%EEP组大鼠胸腺和淋巴结淋巴样增生明显减轻，各EEP组大鼠肾小管上皮细胞增生明显减轻，雌性EEP组大鼠肾上腺皮质增生明显减轻。在血液中，所有接受EEP治疗的男性中性粒细胞显著减少，而接受2.5%EEP治疗的女性中性粒细胞显著减少，表明炎症水平较低。服用2.5%EEP的雌性大鼠血液中总胆固醇和甘油三酯水平显著降低。综上所述，在2年饲养实验条件下，EEP无致癌作用，未引起任何脏器明显的组织病理学改变，进一步发挥抗炎、抗肿瘤作用，提高了Wistar Hnorver大鼠的存活率。
The present study was conducted over a course of 104 weeks to estimate the carcinogenicity of ethanol-extracted Brazilian green propolis (EEP). Groups of 50 male and 50 female Wistar Hannover rats, 6-week-old at commencement were exposed to EEP at doses of 0, 0.5 or 2.5% in the diet. Survival rates of 0.5% and 2.5% EEP-treated male and female rats, respectively, were significantly higher than those of respective control groups. Overall histopathological evaluation of neoplasms in rat tissues after 2 years showed no significant increase of tumors or preneoplastic lesions in any organ of animals administered EEP. Significantly lower incidences of pituitary tumors in 0.5% EEP male and 2.5% EEP female groups, malignant lymphoma/leukemia in both 2.5% EEP-treated males and females and total thyroid tumors in 0.5% EEP male group were found. Administration of EEP caused significant decreases of lymphoid hyperplasia of the thymus and lymph nodes in 2.5% EEP-treated rats, tubular cell hyperplasia of kidneys in all EEP groups, and cortical hyperplasia of adrenals in EEP-treated females. In the blood, significant reduction of neutrophils in all EEP-treated males and band neutrophils in 2.5% EEP-treated females was found indicating lower levels of inflammation. Total cholesterol and triglicerides levels were significantly lower in the blood of 2.5% EEP-treated female rats. In conclusion, under the conditions of the 2-year feeding experiment, EEP was not carcinogenic, did not induce significant histopathological changes in any organ, and further exerted anti-inflammatory and antitumorigenic effects resulting in increase of survival of Wistar Hannover rats.