Are the genes nadA and norB involved in formation of aflatoxin G(1)?
Are the genes nadA and norB involved in formation of aflatoxin G(1)?
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DOI：
10.3390/ijms9091717
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发表时间：
2008-09
影响因子：
5.6
通讯作者：
Chang, Perng-Kuang
中科院分区：
文献类型：
--
作者：
Ehrlich, Kenneth C.;Scharfenstein, Leslie L., Jr.;Montalbano, Beverly G.;Chang, Perng-Kuang
关键词：
黄曲霉毒素是真菌次级代谢产物中毒性最大、致癌性最强的一类，是人类食用食品中常见的污染物。先前的研究表明，由某些曲霉属物种从O-甲基杂色曲霉素（OMST）形成G-组黄曲霉毒素（AF）涉及细胞色素P450单加氧酶OrdA（AflQ）和CypA（AflU）的氧化。然而，转换中的一些步骤尚未完全确定。OYE-FMN结合结构域还原酶编码基因nadA（aflY）的寄生曲霉破坏突变体的提取物含有386 Da AFG 1前体。具有该质量的化合物被预测为OMST的顺序OrdA和CypA氧化的产物。增加量的362 Da的醇，推定产品的NadA减少，积累在真菌提取物与破坏芳基醇脱氢酶编码基因norB。这些结果表明AFG 1的生物合成涉及NadA还原和NorB氧化。
Aflatoxins, the most toxic and carcinogenic family of fungal secondary metabolites, are frequent contaminants of foods intended for human consumption. Previous studies showed that formation of G-group aflatoxins (AFs) from O-methylsterigmatocystin (OMST) by certain Aspergillus species involves oxidation by the cytochrome P450 monooxygenases, OrdA (AflQ) and CypA (AflU). However, some of the steps in the conversion have not yet been fully defined. Extracts of Aspergillus parasiticus disruption mutants of the OYE-FMN binding domain reductase-encoding gene nadA (aflY) contained a 386 Da AFG1 precursor. A compound with this mass was predicted as the product of sequential OrdA and CypA oxidation of OMST. Increased amounts of a 362 Da alcohol, the presumptive product of NadA reduction, accumulate in extracts of fungi with disrupted aryl alcohol dehydrogenase-encoding gene norB. These results show that biosynthesis of AFG1 involves NadA reduction and NorB oxidation.
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