The structure of the lantibiotic lacticin 481 produced by Lactococcus lactis: Location of the thioether bridges
The structure of the lantibiotic lacticin 481 produced by Lactococcus lactis: Location of the thioether bridges
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DOI：
10.1016/0014-5793(96)00771-5
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发表时间：
1996-08-12
期刊：
影响因子：
3.5
通讯作者：
Rollema, HS
中科院分区：
文献类型：
--
作者：
vandenHooven, HW;Lagerwerf, FM;Rollema, HS
lantibiotic lacticin 481是由乳酸乳球菌乳酸亚种（Lactococcus lactis ssp，lactis）产生的一种细菌素，该多肽含有27个氨基酸，包括不常见的脱氢丁酸残基和硫醚桥连的羊毛硫氨酸和3-甲基羊毛硫氨酸。Lacticin 481是一种结构独特的羊毛硫抗生素，它还包括链球菌素A-FF 22、唾液素A和变应霉素。乳酸菌素481中硫醚桥的确切位置通过肽化学、质谱和NMR光谱的组合来确定，显示残基9和14、11和25以及18和26之间的连接。
The lantibiotic lacticin 481 is a bacteriocin produced by Lactococcus lactis ssp, lactis, This polypeptide contains 27 amino acids, including the unusual residues dehydrobutyrine and the thioether-bridging lanthionine and 3-methyllanthionine. Lacticin 481 belongs to a structurally distinct group of lantibiotics, which also include streptococcin A-FF22, salivaricin A and variacin, Here we report the first complete structure of this type of lantibiotic. The exact location of the thioether bridges in lacticin 481 was determined by a combination of peptide chemistry, mass spectrometry and NMR spectroscopy, showing connections between residues 9 and 14, 11 and 25, and 18 and 26.