Identification and quantitation of electron-transport components in human polymorphonuclear neutrophils.
Identification and quantitation of electron-transport components in human polymorphonuclear neutrophils.
复制标题
人多形核中性粒细胞中电子传输成分的鉴定和定量。
DOI：
10.1016/0005-2728(82)90057-3
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Shirley,PS
中科院分区：
文献类型：
--
作者：
Cunningham,CC;DeChatelet,LR;Spach,PI;Parce,JW;Thomas,MJ;Lees,CJ;Shirley,PS
使用连二亚硫酸盐差光谱，我们已经检测到细胞色素高度纯化的人中性粒细胞在0.08 nmol/mg蛋白质的浓度。在脂质提取物中鉴定出醌的存在，浓度约为100 μ g/ml。0.06 nmol/mg蛋白质。经质谱分析鉴定为泛醌-10。细胞色素氧化酶的同时测量表明，这些化合物不能归因于线粒体污染。这些结果是兼容的假设，即在人类中性粒细胞呼吸爆发的启动涉及多组分电子传递系统。
Using dithionite difference spectra we have detected cytochromebin highly purified human neutrophils at a concentration of 0.08 nmol/mg protein. The presence of quinone was identified in lipid extracts at a concentration of approx. 0.06 nmol/mg protein. It was identified as ubiquinone-10 by mass spectrographic analysis. Simultaneous measurements of cytochrome oxidase indicated that these compounds could not be attributed to mitochondrial contamination. These results are compatible with the hypothesis that initiation of the respiratory burst in human neutrophils involves a multicomponent electron-transport system.