Inhibitory effects of polysaccharides isolated from Phellinus gilvus on benzo(a)pyrene-induced forestomach carcinogenesis in mice
Inhibitory effects of polysaccharides isolated from Phellinus gilvus on benzo(a)pyrene-induced forestomach carcinogenesis in mice
复制标题
DOI：
10.3748/wjg.v11.i4.577
复制
发表时间：
2005-01-28
影响因子：
4.3
通讯作者：
Jin, Hee-Kyung
中科院分区：
文献类型：
--
作者：
Bae, Jae-Sung;Jang, Kwang-Ho;Jin, Hee-Kyung
目的：桑黄多糖具有抗肿瘤活性，但目前尚无关于桑黄多糖抗肿瘤作用的报道。本实验研究了PG多糖对苯并(A)芘(BaP)诱发ICR雌性小鼠前胃癌变的调节作用。方法：40只ICR雌性小鼠灌胃给予BaP 4wk，建立前胃癌模型。将小鼠随机分为4组，每组10只。每组小鼠分别用无菌水或PG处理4wk和8wk(SW4、PGW4、SW8和PGW8组)。结果：在组织病理学和RT-PCR分析中，无菌水处理组小鼠胃粘膜明显增生，突变型P53基因表达明显增强。结论：PG多糖可能通过下调突变型P53蛋白的表达来抑制BaP诱导的小鼠前胃癌的发生。(C)2005年WJG Press和Elsevier Inc.保留所有权利。
AIM: Although polysaccharides from Phellinus mushrooms are a well-known material with anti-tumor properties, there is no information about the effect of polysaccharides from Phellinus gilvus (PG) on tumor. The modulating effect of polysaccharides isolated from PG on the benzo(a) pyrene (BaP)-induced forestomach carcinogenesis in ICR female mice was investigated in this study.METHODS: A forestomach carcinogenesis model was established in 40 ICR female mice receiving oral administration of BaP for 4 wk. The mice were randomly assigned to 4 groups (10 each). The mice in each group were treated with sterile water or PG for 4 and 8 wk (SW4, PGW4, SW8, and PGW8 groups). Eight or 12 wk after the first dose of BaP, forestomachs were removed for histopathological and RT-PCR analysis.RESULTS: In histopathological changes and RT-PCR analysis, sterile water-treated mice showed significant hyperplasia of the gastric mucosa with a significantly increased expression of mutant p53 mRNA compared to mice treated with PG for 8 wk.CONCLUSION: Polysaccharides isolated from PG may inhibit BaP-induced forestomach carcinogenesis in mice bydown-regulating mutant p53 expression. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.