Molecular and cellular organization of the taste system in the Drosophila larva.
Molecular and cellular organization of the taste system in the Drosophila larva.
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DOI：
10.1523/jneurosci.3363-11.2011
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发表时间：
2011-10-26
期刊：
影响因子：
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通讯作者：
Carlson JR
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文献类型：
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作者：
Kwon JY;Dahanukar A;Weiss LA;Carlson JR
我们通过全面分析所有 68 种味觉受体 (Grs) 的表达模式，研究果蝇幼虫味觉感知的分子和细胞基础。对代表每个 Gr 的 Gr-GAL4 系进行了检查，发现 39 个在幼虫头部的味觉器官中表达，包括末端器官 (TO)、背器官 (DO) 和咽器官。构建了受体到神经元图。该图谱定义了 TO 和 DO 的 10 个神经元，并识别了映射到它们的 28 个受体。除了表达相同补体的两个神经元之外，每个神经元都表达 Gr-GAL4 驱动程序的独特子集。所有这些神经元都至少表达 2 个驱动因子，其中一个神经元表达 17 个驱动因子。许多受体仅映射到这些细胞之一，但有些受体映射到多达 6 个。幼虫中表达的 Gr-GAL4 驱动程序名册中明显缺少的是糖受体亚家族的驱动程序。共表达分析表明，大多数幼虫 Grs 都会产生苦味反应，并且存在独特的苦味感受神经元。对中心投影的综合分析证实了从不同区域收集的感官信息，例如头尖与咽部的处理过程是在食管下神经节（SOG）的不同区域进行的，SOG是中枢神经系统的主要味觉中枢。总而言之，这些结果提供了幼虫味觉系统的分子和细胞组织的广泛视图。
We examine the molecular and cellular basis of taste perception in the Drosophila larva, through a comprehensive analysis of the expression patterns of all 68 Gustatory receptors (Grs). Gr-GAL4 lines representing each Gr are examined, and 39 show expression in taste organs of the larval head, including the terminal organ (TO), the dorsal organ (DO), and the pharyngeal organs. A receptor-to-neuron map is constructed. The map defines 10 neurons of the TO and DO, and it identifies 28 receptors that map to them. Each of these neurons expresses a unique subset of Gr-GAL4 drivers, except for two neurons that express the same complement. All of these neurons express at least two drivers, and one neuron expresses 17. Many of the receptors map to only one of these cells, but some map to as many as six. Conspicuously absent from the roster of Gr-GAL4 drivers expressed in larvae are those of the sugar receptor subfamily. Coexpression analysis suggests that most larval Grs act in bitter response, and that there are distinct bitter-sensing neurons. A comprehensive analysis of central projections confirms that sensory information collected from different regions, e.g. the tip of the head vs the pharynx, is processed in different regions of the suboesophageal ganglion (SOG), the primary taste center of the central nervous system. Taken together, the results provide an extensive view of the molecular and cellular organization of the larval taste system.