Detection of lymph node involvement in hematologic malignancies using micromagnetic resonance lymphangiography with a gadolinum-labeled dendrimer nanoparticle
Detection of lymph node involvement in hematologic malignancies using micromagnetic resonance lymphangiography with a gadolinum-labeled dendrimer nanoparticle
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DOI：
10.1593/neo.05454
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发表时间：
2005-11-01
期刊：
影响因子：
4.8
通讯作者：
Choyke, PL
中科院分区：
文献类型：
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作者：
Kobayashi, H;Kawamoto, S;Choyke, PL
淋巴瘤的动物模型应该反映它们在人类中的对应物;然而，基于直接可视化，可能难以确定诱导的疾病是淋巴内还是淋巴外。目前的影像学方法不足以识别淋巴和淋巴内受累。为了区分淋巴管内淋巴管外受累，我们开发了一种基于磁共振成像的淋巴管造影方法，并在两种淋巴瘤动物模型上进行了测试。将钆（Gd）标记的树枝状聚合物纳米颗粒（第6代;类似于220 kDa/类似于10 nm）连续注射到携带血液恶性肿瘤的小鼠中以进行动态微磁共振淋巴管造影术（micro-MRIL）。在一项成像研究中，比较了标准T1加权3D快速扰相梯度回波和采用稳态采集的T2/T1加权D快速成像（3D-FIESTA-C），以区分淋巴管内和淋巴管外肿瘤累及。两种方法均能显示淋巴结和淋巴结。此外，3D-FIESTA-C描绘了淋巴系统和淋巴外肿瘤。在动物模型中，3D-FIESTA-C证明了被认为是淋巴内的肿瘤的大部分实际上是淋巴外的。总之，微MRL，使用Gd标记的树枝状聚合物纳米颗粒与组合的方法，可以定义正常和异常的淋巴系统，并可以区分淋巴内和淋巴外疾病的小鼠恶性淋巴瘤模型。
Animal models of lymphoma should reflect their counterparts in humans; however, it can be difficult to ascertain whether an induced disease is intralymphatic or extralymphatic based on direct visualization. Current imaging methods are insufficient for identifying lymphatic and intralymphatic involvement. To differentiate intralymphatic from extralymphatic involvement, we have developed a magnetic resonance imaging-based lymphangiography method and tested it on two animal models of lymphoma. A gadolinium (Gd)-labeled dendrimer nanoparticle (generation-6; similar to 220 kDa/similar to 10 nm) was injected interstitially into mice bearing hematologic malignancies to perform dynamic micromagnetic resonance lymphangiography (micro-MRIL). Both a standard T1-weighted 3D fast spoiled gradient echo and a T2/T1-weighted D-fast imaging employing steady-state acquisition (3D-FIESTA-C) were compared in an imaging study to differentiate intralymphatic from extralymphatic involvement of tumors. The lymphatics and lymph nodes were visualized with both methods in all cases. In addition, 3D-FIESTA-C depicted both the lymphatic system and the extralymphatic tumor. In an animal model, 3D-FIESTA-C demonstrated that the bulk of the tumor thought to be intralymphatic was actually extralymphatic. In conclusion, micro-MRL, using Gd-labeled dendrimer nanoparticles with the combined method, can define both the normal and abnormal lymphatics and can distinguish intralymphatic from extralymphatic diseases in mouse models of malignant lymphoma.