Observation of single-stranded DNA on mica and highly oriented pyrolytic graphite by atomic force microscopy
Observation of single-stranded DNA on mica and highly oriented pyrolytic graphite by atomic force microscopy
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DOI：
10.1016/j.febslet.2006.09.017
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发表时间：
2006-10-16
期刊：
影响因子：
3.5
通讯作者：
Dietler, Giovanni
中科院分区：
文献类型：
--
作者：
Adamcik, Jozef;Klinov, Dmitry V.;Dietler, Giovanni
采用原子力显微镜对单链DNA （ssDNA）吸附在Mg2+修饰的云母、3-氨基丙基三乙氧基硅烷或修饰的高取向热解石墨（HOPG）上进行了成像。云母上的ssDNA分子结构紧凑，具有块状、环状和超捻等结构，而改性HOPG石墨上的ssDNA分子则没有二级结构。我们已经证明，在标准条件下将ssDNA固定在修饰的HOPG上可以消除分子内碱基配对，因此这种方法对于更详细地研究涉及ssDNA的某些过程可能是重要的。(c) 2006年欧洲生化学会联合会。Elsevier B.V.版权所有。
Atomic force microscopy was used to image single-stranded DNA (ssDNA) adsorbed on mica modified by Mg2+, by 3-aminopropyltriethoxysilane or on modified highly oriented pyrolytic graphite (HOPG). ssDNA molecules on mica have compact structures with lumps, loops and super twisting, while on modified HOPG graphite ssDNA molecules adopt a conformation without secondary structures. We have shown that the immobilization of ssDNA under standard conditions on modified HOPG eliminates intramolecular base-pairing, thus this method could be important for studying certain processes involving ssDNA in more details. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.