Efficacy of topical formulations containing Pimenta pseudocaryophyllus extract against UVB-induced oxidative stress and inflammation in hairless mice
Efficacy of topical formulations containing Pimenta pseudocaryophyllus extract against UVB-induced oxidative stress and inflammation in hairless mice
复制标题
DOI：
10.1016/j.jphotobiol.2013.08.007
复制
发表时间：
2013-10-05
影响因子：
5.4
通讯作者：
Casagrande, Rubia
中科院分区：
文献类型：
--
作者：
Campanini, Marcela Z.;Pinho-Ribeiro, Felipe A.;Casagrande, Rubia
富含抗氧化物质的植物可能是预防紫外线诱导的皮肤氧化和炎症损伤的一种有前途的策略。假香石竹原产于巴西，具有高浓度的黄酮类化合物和其他多酚化合物。因此，本研究评估了含有假香石竹乙醇提取物（PPE）的局部制剂在抑制UV-B辐射诱导的氧化应激和炎症方面的可能作用。在UV-B照射之前和之后，使用两种制剂：F1（高脂质含量的非离子乳剂）和F2（低脂质含量的阴离子乳剂）在无毛小鼠的背部皮肤上施用PPE。在皮肤样本中评价以下参数：水肿、髓过氧化物酶活性、细胞因子水平、基质金属蛋白酶-9（MMP-9）分泌/活性、还原型谷胱甘肽（GSH）、超氧阴离子和脂质过氧化水平以及谷胱甘肽还原酶和gp 91 phox的mRNA表达。UV-B辐射增加了所有参数，除了IL-10水平和谷胱甘肽还原酶mRNA的表达，这是没有改变，和GSH水平，这是减少暴露于UV-B光。用含有PPE的F1和F2处理抑制UV-B诱导的水肿形成（89%和86%），髓过氧化物酶活性（85%和81%），IL-1 β产生（62%和82%），MMP-9活性（71%和74%），GSH耗竭（73%和85%），超氧阴离子（83%和66%）和TBARS（100%和100%）水平，增加谷胱甘肽还原酶（2.54和2.55倍）和减少gp 91 phox（67%和100%）mRNA表达。含有PPE的F2也增加IL-10水平。因此，本研究证明了含有PPE的局部制剂在抑制UV-B辐射诱导的皮肤炎症和氧化应激方面的有效性。(C)2013爱思唯尔有限公司版权所有。
Plants rich in antioxidant substances may be a promising strategy for preventing UV-induced oxidative and inflammatory damage of the skin. Pimenta pseudocaryophyllus is native to Brazil and presents flavonoids and other polyphenolic compounds in high concentration. Thus, the present study evaluated the possible effects of topical formulations containing P. pseudocaryophyllus ethanolic extract (PPE) at inhibiting UV-B irradiation-induced oxidative stress and inflammation. PPE was administered on the dorsal skin of hairless mice using two formulations: F1 (non-ionic emulsion with high lipid content) and F2 (anionic emulsion with low lipid content) before and after UV-B irradiation. The following parameters were evaluated in skin samples: edema, myeloperoxidase activity, cytokines levels, matrix metalloprotease-9 (MMP-9) secretion/activity, reduced glutathione (GSH), superoxide anion and lipid peroxidation levels, and mRNA expression for glutathione reductase and gp91phox. The UV-B irradiation increased all parameters, except for IL-10 levels and glutathione reductase mRNA expression, which were not altered, and GSH levels, which were reduced by exposure to UV-B light. Treatments with F1 and F2 containing PPE inhibited UV-B-induced edema formation (89% and 86%), myeloperoxidase activity (85% and 81%), IL-1 beta production (62% and 82%), MMP-9 activity (71% and 74%), GSH depletion (73% and 85%), superoxide anion (83% and 66%) and TBARS (100% and 100%) levels, increased glutathione reductase (2.54 and 2.55-fold) and reduced gp91phox (67% and 100%) mRNA expression, respectively. F2 containing PPE also increased IL-10 levels. Therefore, this study demonstrates the effectiveness of topical formulations containing PPE in inhibiting UV-B irradiation-induced inflammation and oxidative stress of the skin. (C) 2013 Elsevier B.V. All rights reserved.